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β-Adaptin Antibody (A-5): sc-74423
- β-Adaptin Antibody (A-5) is a mouse monoclonal IgG2a κ β-Adaptin antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 650-949 of β1-Adaptin of human origin
- beta-Adaptin Antibody (A-5) is recommended for detection of β1-Adaptin and β2-Adaptin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-beta-Adaptin Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Adaptin (A-5): sc-74423.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for β-Adaptin Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with β-Adaptin Antibody (A-5) (see ordering information below).
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β-Adaptin Antibody (A-5) is an IgG2a κ mouse monoclonal beta-Adaptin antibody (also designated β-Adaptin antibody) that detects the beta-Adaptin protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. β-Adaptin Antibody (A-5) is available as both the non-conjugated anti-beta-Adaptin antibody form, as well as multiple conjugated forms of anti-beta-Adaptin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of clathrin binding adaptin proteins (g and β1 for AP-1, α and β2 for AP-2) and two smaller subunits known as AP50 and AP17 (2-4). The alpha and beta adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length. α-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues (5,6). AP180 (also designated AP-3 or F1-20) is a synapse-specific clathrin assembly protein. The protein CALM (clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in clathrin assembly..
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Conservation and diversity in families of coated vesicle adaptins. | Ponnambalam, S., et al. 1990. J Biol Chem. 265: 4814-20. PMID: 1969413
- Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus. | Robinson, MS. 1990. J Cell Biol. 111: 2319-26. PMID: 2126014
- Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2. | Kirchhausen, T., et al. 1989. Proc Natl Acad Sci U S A. 86: 2612-6. PMID: 2495531
- Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins). | Robinson, MS. 1989. J Cell Biol. 108: 833-42. PMID: 2564002
- Expression and localization of alpha-adaptin isoforms. | Ball, CL., et al. 1995. J Cell Sci. 108 (Pt 8): 2865-75. PMID: 7593326
- Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site. | Morris, SA., et al. 1993. EMBO J. 12: 667-75. PMID: 8440257
- The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family. | Dreyling, MH., et al. 1996. Proc Natl Acad Sci U S A. 93: 4804-9. PMID: 8643484
- Endocytosis and molecular sorting. | Mellman, I. 1996. Annu Rev Cell Dev Biol. 12: 575-625. PMID: 8970738
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β-Adaptin Antibody (A-5) | sc-74423 | 200 µg/ml | $310.00 | |||
β-Adaptin Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-528664 | 200 µg Ab; 10 µg BP | $348.00 | |||
β-Adaptin Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-521109 | 200 µg Ab, 40 µg BP | $348.00 | |||
β-Adaptin Antibody (A-5) AC | sc-74423 AC | 500 µg/ml, 25% agarose | $408.00 | |||
β-Adaptin Antibody (A-5) HRP | sc-74423 HRP | 200 µg/ml | $310.00 | |||
β-Adaptin Antibody (A-5) FITC | sc-74423 FITC | 200 µg/ml | $324.00 | |||
β-Adaptin Antibody (A-5) PE | sc-74423 PE | 200 µg/ml | $337.00 | |||
β-Adaptin Antibody (A-5) Alexa Fluor® 488 | sc-74423 AF488 | 200 µg/ml | $350.00 | |||
β-Adaptin Antibody (A-5) Alexa Fluor® 546 | sc-74423 AF546 | 200 µg/ml | $350.00 | |||
β-Adaptin Antibody (A-5) Alexa Fluor® 594 | sc-74423 AF594 | 200 µg/ml | $350.00 | |||
β-Adaptin Antibody (A-5) Alexa Fluor® 647 | sc-74423 AF647 | 200 µg/ml | $350.00 | |||
β-Adaptin Antibody (A-5) Alexa Fluor® 680 | sc-74423 AF680 | 200 µg/ml | $350.00 | |||
β-Adaptin Antibody (A-5) Alexa Fluor® 790 | sc-74423 AF790 | 200 µg/ml | $350.00 |