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Western blot analysis of Dynamin I phosphorylation in untreated (A,D), Ser/Thr Phosphorylation Induction Cocktail (sc-362324) treated (B,E) and Ser/Thr Phosphorylation Induction Cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (CF) SH-SY5Y whole cell lysates. Antibodies tested include p-Dynamin I Antibody (E-9): sc-377563 (A,B,C) and Dynamin I (D5): sc-12724 (D,E,F).
p-Dynamin I Antibody (E-9): sc-377563
- p-Dynamin I Antibody (E-9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids Ser 795 Ser 795 of Dynamin I of human origin
- recommended for detection of Ser 795 phosphorylated Dynamin I of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p-Dynamin I Antibody (E-9) for WB applications. These reagents are now offered in bundles with p-Dynamin I Antibody (E-9) (see ordering information below).
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Description
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Research Information
Dynamin I is a GTPase enzyme required for the retrieval of synaptic vesicles after exocytosis and functions in endocytosis by stimulating assembly of invaginating synaptic vesicles. Dynamin I is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C (PKC) (2-5). The phosphorylation site in PKC-phosphorylated Dynamin I is a single site at Serine 795, which is located near a binding site for the SH3 domain of p85, the regulatory subunit of phosphatidylinositol 3-kinase (2-5). Dephosphorylation is required for synaptic vesicle retrieval, suggesting that phosphorylation affects the subcellular localization of Dynamin I.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Dynamin I Antibody (E-9) | sc-377563 | 200 µg/ml | $310.00 | |||
p-Dynamin I Antibody (E-9): m-IgG Fc BP-HRP Bundle | sc-540736 | 200 µg Ab; 10 µg BP | $348.00 | |||
p-Dynamin I Antibody (E-9): m-IgG1 BP-HRP Bundle | sc-542335 | 200 µg Ab; 20 µg BP | $348.00 | |||
p-Dynamin I (E-9) Neutralizing Peptide | sc-377563 P | 100 µg/0.5 ml | $67.00 |