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StAR Antibody (D-2): sc-166821
- StAR Antibody (D-2) is a mouse monoclonal IgG1 κ StAR antibody, cited in 31 publications, provided at 200 µg/ml
- raised against amino acids 1-285 representing full length StAR of human origin
- StAR Antibody (D-2) is recommended for detection of StAR of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-StAR Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of StAR (D-2): sc-166821.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for StAR Antibody (D-2) for WB applications. These reagents are now offered in bundles with StAR Antibody (D-2) (see ordering information below).
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SEE ALSO...
StAR Antibody (D-2) is an IgG1 κ mouse monoclonal StAR antibody (also designated STARD1 antibody, StAR related lipid transfer (START) domain containing 1 antibody, or steroidogenic acute regulatory protein antibody) that detects the StAR protein of mouse, rat and human origin by WB, IP, IF and ELISA. StAR Antibody (D-2) is available as both the non-conjugated anti-StAR antibody form, as well as multiple conjugated forms of anti-StAR antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Steroidogenic acute regulatory (StAR) protein appears to mediate the rapid increase in pregnenolone synthesis stimulated by tropic hormones. StAR increases pregnenolone synthesis more than 4-fold and a major StAR transcript of 1.6 kb is found in ovary and testis. During ongoing growth and differentiation of the follicle of the ovary, the immunoreactivity of StAR tends to shift from the granulosa cells of early antral follicles to the theca cell layers in the adult (2-4). The first and rate-limiting step of steroidogenesis is the transfer of cholesterol from the outer mitochondrial membrane to the inner membrane where it is converted to pregnenolone by cytochrome P450 side-chain cleavage. This reaction is modulated in the gonads and adrenals by StAR, however, the mechanism used by StAR is not understood. This protein was isolated from a human adrenal cortex library and nonsense mutations in the StAR gene can cause lipoid congenital adrenal hyperplasia. The gene which encodes StAR maps to human chromosome 8p11.2.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Immunolocalization and expression of the steroidogenic acute regulatory protein during the transitional stages of rat follicular differentiation. | Thompson, WE., et al. 1999. J Histochem Cytochem. 47: 769-76. PMID: 10330453
- Does cholesterol use the mitochondrial contact site as a conduit to the steroidogenic pathway? | Thomson, M. 2003. Bioessays. 25: 252-8. PMID: 12596229
- Human steroidogenic acute regulatory protein: functional activity in COS-1 cells, tissue-specific expression, and mapping of the structural gene to 8p11.2 and a pseudogene to chromosome 13. | Sugawara, T., et al. 1995. Proc Natl Acad Sci U S A. 92: 4778-82. PMID: 7761400
- Role of steroidogenic acute regulatory protein in adrenal and gonadal steroidogenesis. | Lin, D., et al. 1995. Science. 267: 1828-31. PMID: 7892608
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
StAR Antibody (D-2) | sc-166821 | 200 µg/ml | $310.00 | |||
StAR Antibody (D-2): m-IgG Fc BP-HRP Bundle | sc-529059 | 200 µg Ab; 10 µg BP | $348.00 | |||
StAR Antibody (D-2): m-IgGκ BP-HRP Bundle | sc-521758 | 200 µg Ab, 40 µg BP | $348.00 | |||
StAR Antibody (D-2): m-IgG1 BP-HRP Bundle | sc-543295 | 200 µg Ab; 20 µg BP | $348.00 | |||
StAR Antibody (D-2) AC | sc-166821 AC | 500 µg/ml, 25% agarose | $408.00 | |||
StAR Antibody (D-2) HRP | sc-166821 HRP | 200 µg/ml | $310.00 | |||
StAR Antibody (D-2) FITC | sc-166821 FITC | 200 µg/ml | $324.00 | |||
StAR Antibody (D-2) PE | sc-166821 PE | 200 µg/ml | $337.00 | |||
StAR Antibody (D-2) Alexa Fluor® 488 | sc-166821 AF488 | 200 µg/ml | $350.00 | |||
StAR Antibody (D-2) Alexa Fluor® 546 | sc-166821 AF546 | 200 µg/ml | $350.00 | |||
StAR Antibody (D-2) Alexa Fluor® 594 | sc-166821 AF594 | 200 µg/ml | $350.00 | |||
StAR Antibody (D-2) Alexa Fluor® 647 | sc-166821 AF647 | 200 µg/ml | $350.00 | |||
StAR Antibody (D-2) Alexa Fluor® 680 | sc-166821 AF680 | 200 µg/ml | $350.00 | |||
StAR Antibody (D-2) Alexa Fluor® 790 | sc-166821 AF790 | 200 µg/ml | $350.00 |