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15-LO Antibody (B-7): sc-133085
- 15-LO Antibody (B-7) is a mouse monoclonal IgG1 κ 15-LO antibody, cited in 20 publications, provided at 200 µg/ml
- raised against acids 428-662 mapping at the C-terminus of 15-LO of human origin
- 15-LO Antibody (B-7) is recommended for detection of 15-LO and leukocyte-, platelet- and epidermal-type 12-LO of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-15-LO Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of 15-LO (B-7): sc-133085.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for 15-LO Antibody (B-7) for WB applications. These reagents are now offered in bundles with 15-LO Antibody (B-7) (see ordering information below).
15-LO Antibody (B-7) is an IgG1 κ mouse monoclonal 15-LO antibody (also designated LOX15 antibody, Arachidonate 15-Lipoxygenase antibody, 15-LOX-1 antibody, Polyunsaturated Fatty Acid Lipoxygenase ALOX15 antibody, Arachidonate 12-Lipoxygenase Leukocyte-Type antibody, Arachidonate Omega-6 Lipoxygenase antibody, Hepoxilin A3 Synthase Alox15 antibody, 15-LOX antibody, LOG15 antibody, 15-Lipoxygenase Type 1 antibody, or 15-Lipooxygenase-1 antibody) that detects the 15-LO protein of mouse, rat and human origin by WB, IP, IF and ELISA. 15-LO Antibody (B-7) is available as both the non-conjugated anti-15-LO antibody form, as well as multiple conjugated forms of anti-15-LO antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Lipoxygenases are a family of enzymes which dioxygenate unsaturated fatty acids, thus initiating lipoperoxidation of membranes and synthesis of signaling molecules, as well as inducing structural and metabolic changes in the cell. The Lox enzymes in mammals include 12-LO and 15-LO, which are classified with respect to their positional specificity of the deoxygenation of their most common substrate, arachidonic acid. The metabolism of arachidonic acid leads to the generation of biologically active metabolites that have been implicated in cell growth and proliferation, as well as survival and apoptosis. 15-Lipoxygenase (15-LO) acts in physiological membrane remodeling and the pathogenesis of atherosclerosis, inflammation, and carcinogenesis. It is highly regulated and expressed in a tissue- and cell-type-specific fashion. IL-4 and IL-13 play important roles in transactivating the 15-LO gene. Overexpression of 15-LO type 1 in prostate cancer contributes to the cancer progression by regulating IGF-1R expression and activation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Overexpression of platelet-type 12-lipoxygenase promotes tumor cell survival by enhancing alpha(v)beta(3) and alpha(v)beta(5) integrin expression. | Pidgeon, GP., et al. 2003. Cancer Res. 63: 4258-67. PMID: 12874035
- 15-lipoxygenase-1 expression upregulates and activates insulin-like growth factor-1 receptor in prostate cancer cells. | Kelavkar, UP. and Cohen, C. 2004. Neoplasia. 6: 41-52. PMID: 15068670
- Transcriptional regulation of 15-lipoxygenase expression by promoter methylation. | Liu, C., et al. 2004. Exp Cell Res. 297: 61-7. PMID: 15194425
- Molecular identification, localization and function of platelet-type 12-lipoxygenase in human melanoma progression, under experimental and clinical conditions. | Rásó, E., et al. 2004. Melanoma Res. 14: 245-50. PMID: 15305153
- Aspirin enhances the sensitivity of human platelet 12-lipoxygenase to inhibition by 15-HETE, an endogenous regulator. | Fletcher-Cieutat, M., et al. 1985. Prostaglandins Leukot Med. 18: 255-9. PMID: 3925465
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
15-LO Antibody (B-7) | sc-133085 | 200 µg/ml | $310.00 | |||
15-LO Antibody (B-7): m-IgG Fc BP-HRP Bundle | sc-528744 | 200 µg Ab; 10 µg BP | $348.00 | |||
15-LO Antibody (B-7): m-IgGκ BP-HRP Bundle | sc-521227 | 200 µg Ab, 40 µg BP | $348.00 | |||
15-LO Antibody (B-7): m-IgG1 BP-HRP Bundle | sc-543108 | 200 µg Ab; 20 µg BP | $348.00 | |||
15-LO Antibody (B-7) AC | sc-133085 AC | 500 µg/ml, 25% agarose | $408.00 | |||
15-LO Antibody (B-7) HRP | sc-133085 HRP | 200 µg/ml | $310.00 | |||
15-LO Antibody (B-7) FITC | sc-133085 FITC | 200 µg/ml | $324.00 | |||
15-LO Antibody (B-7) PE | sc-133085 PE | 200 µg/ml | $337.00 | |||
15-LO Antibody (B-7) Alexa Fluor® 488 | sc-133085 AF488 | 200 µg/ml | $350.00 | |||
15-LO Antibody (B-7) Alexa Fluor® 546 | sc-133085 AF546 | 200 µg/ml | $350.00 | |||
15-LO Antibody (B-7) Alexa Fluor® 594 | sc-133085 AF594 | 200 µg/ml | $350.00 | |||
15-LO Antibody (B-7) Alexa Fluor® 647 | sc-133085 AF647 | 200 µg/ml | $350.00 | |||
15-LO Antibody (B-7) Alexa Fluor® 680 | sc-133085 AF680 | 200 µg/ml | $350.00 | |||
15-LO Antibody (B-7) Alexa Fluor® 790 | sc-133085 AF790 | 200 µg/ml | $350.00 |