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ADH Antibody (G-7): sc-133207
- ADH Antibody (G-7) is a mouse monoclonal IgG2a κ ADH antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of ADH of human origin
- ADH Antibody (G-7) is recommended for detection of ADHα, ADHβ and ADHγ of human origin and Adh1 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ADH Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADH (G-7): sc-133207.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ADH Antibody (G-7) for WB and IHC(P) applications. These reagents are now offered in bundles with ADH Antibody (G-7) (see ordering information below).
ADH Antibody (G-7) is an IgG2a κ mouse monoclonal ADH antibody (also designated ADH antibody) that detects the ADH protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. ADH Antibody (G-7) is available as both the non-conjugated anti-ADH antibody form, as well as multiple conjugated forms of anti-ADH antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha (ADH1A), beta (ADH1B) and gamma (ADH1C) subunits are tandemly organized on chromosome 4q22 as a gene cluster. The alpha form of ADH is monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. The genes encoding beta and gamma subunits, however, are polymorphic and strongly expressed in adult livers. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH in the liver is the removal of ethanol formed by microorganisms in the intestinal tract.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Alcohol dehydrogenase (ADH) isoenzyme activity in the sera of patients with Helicobacter pylori infection. | Jelski, W., et al. 2007. Dig Dis Sci. 52: 1513-6. PMID: 17404875
- Chromosomal assignment of the alcohol dehydrogenase cluster locus to human chromosome 4q21-23 by in situ hybridization. | Tsukahara, M. and Yoshida, A. 1989. Genomics. 4: 218-20. PMID: 2737681
- Studies on the subunit structure and molecular size of the human alcohol dehydrogenase isozymes determined by the different loci, ADH1, ADH2, and ADH3. | Smith, M., et al. 1973. Ann Hum Genet. 36: 401-14. PMID: 4748759
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ADH Antibody (G-7) | sc-133207 | 200 µg/ml | $310.00 | |||
ADH Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-528769 | 200 µg Ab; 10 µg BP | $348.00 | |||
ADH Antibody (G-7): m-IgGκ BP-HRP Bundle | sc-521275 | 200 µg Ab, 40 µg BP | $348.00 | |||
ADH Antibody (G-7): m-IgG2a BP-HRP Bundle | sc-547165 | 200 µg Ab; 10 µg BP | $348.00 | |||
ADH Antibody (G-7) AC | sc-133207 AC | 500 µg/ml, 25% agarose | $408.00 | |||
ADH Antibody (G-7) HRP | sc-133207 HRP | 200 µg/ml | $310.00 | |||
ADH Antibody (G-7) FITC | sc-133207 FITC | 200 µg/ml | $324.00 | |||
ADH Antibody (G-7) PE | sc-133207 PE | 200 µg/ml | $337.00 | |||
ADH Antibody (G-7) Alexa Fluor® 488 | sc-133207 AF488 | 200 µg/ml | $350.00 | |||
ADH Antibody (G-7) Alexa Fluor® 546 | sc-133207 AF546 | 200 µg/ml | $350.00 | |||
ADH Antibody (G-7) Alexa Fluor® 594 | sc-133207 AF594 | 200 µg/ml | $350.00 | |||
ADH Antibody (G-7) Alexa Fluor® 647 | sc-133207 AF647 | 200 µg/ml | $350.00 | |||
ADH Antibody (G-7) Alexa Fluor® 680 | sc-133207 AF680 | 200 µg/ml | $350.00 | |||
ADH Antibody (G-7) Alexa Fluor® 790 | sc-133207 AF790 | 200 µg/ml | $350.00 |