Aldolase C Antibody (H-11): sc-271593

Aldolase C Antibody (H-11) is an IgG₁ κ mouse monoclonal Aldolase C antibody (also designated ALDOC antibody, ALDC antibody, Fructose-bisphosphate aldolase C antibody, or Brain-type aldolase antibody) that detects the Aldolase C protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Aldolase C Antibody (H-11) is available as both the non-conjugated anti-Aldolase C antibody form, as well as multiple conjugated forms of anti-Aldolase C antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate. Fructose 1,6-bisphosphate aldolase exists as three forms, the muscle-specific Aldolase A, the liver-specific aldolase B, and the brain-specific aldolase C. Aldolase A, B, and C arose from a common ancestral gene, from which aldolase B first diverged. Aldolase A is one of the most highly conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon and has been implicated in hereditary fructose intolerance disease (3,5). Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells (6,8-10). Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells (7,10).

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Exploring substrate binding and discrimination in fructose1, 6-bisphosphate and tagatose 1,6-bisphosphate aldolases.  |  Zgiby, SM., et al. 2000. Eur J Biochem. 267: 1858-68. PMID: 10712619
2. Characterization of the responsive elements to hormones in the rat aldolase B gene.  |  Takano, Y., et al. 2000. Arch Biochem Biophys. 377: 58-64. PMID: 10775441
3. The complete amino acid sequence of human skeletal-muscle fructose-bisphosphate aldolase.  |  Freemont, PS., et al. 1988. Biochem J. 249: 779-88. PMID: 3355497
4. Human aldolase A gene. Structural organization and tissue-specific expression by multiple promoters and alternate mRNA processing.  |  Izzo, P., et al. 1988. Eur J Biochem. 174: 569-78. PMID: 3391172
5. Distribution of Purkinje cell-specific Zebrin-II/aldolase C immunoreactivity in the mouse, rat, rabbit, and human retina.  |  Caffε, AR., et al. 1994. J Comp Neurol. 348: 291-7. PMID: 7814693
6. Aldolase C/zebrin II and the regionalization of the cerebellum.  |  Hawkes, R. and Herrup, K. 1995. J Mol Neurosci. 6: 147-58. PMID: 8672398
7. A search for primitive Purkinje cells: zebrin II expression in sea lampreys (Petromyzon marinus).  |  Lannoo, MJ. and Hawkes, R. 1997. Neurosci Lett. 237: 53-5. PMID: 9406878
8. Genomic sequences of aldolase C (Zebrin II) direct lacZ expression exclusively in non-neuronal cells of transgenic mice.  |  Walther, EU., et al. 1998. Proc Natl Acad Sci U S A. 95: 2615-20. PMID: 9482935
9. The glutamate transporter EAAT4 in rat cerebellar Purkinje cells: a glutamate-gated chloride channel concentrated near the synapse in parts of the dendritic membrane facing astroglia.  |  Dehnes, Y., et al. 1998. J Neurosci. 18: 3606-19. PMID: 9570792
10. Regionalization defects in the weaver mouse cerebellum.  |  Eisenman, LM., et al. 1998. J Comp Neurol. 394: 431-44. PMID: 9590553

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Aldolase C Antibody (H-11) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-Aldolase C Antibody (H-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Aldolase C Antibody (H-11) Specifications

Alternate Product Name

Aldolase C

Product Clone Name

H-11

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

77-112

Epitope Target

Aldolase C

Epitope Species

human

Product Reactivity/Detection

Aldolase C

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

11 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin