Click on image or enlarge button to enlarge
ASL Antibody (E-5): sc-374353
- ASL Antibody (E-5) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1-29 at the N-terminus of ASL of human origin
- Anti-ASL Antibody (E-5) is recommended for detection of ASL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
- Anti-ASL Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ASL (E-5): sc-374353.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ASL Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with ASL Antibody (E-5) (see ordering information below).
QUICK LINKS
SEE ALSO...
ASL Antibody (E-5) is an IgG1 κ mouse monoclonal ASL antibody that detects ASL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. ASL Antibody (E-5) is available as the non-conjugated anti-ASL antibody form, as well as multiple conjugated forms of anti-ASL antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. ASL (argininosuccinate lyase), also known as ASAL or arginosuccinase, is a member of the lyase 1 family of proteins and is predominantly expressed in the liver. Localizing to the cytoplasm and existing as a homotetramer, ASL catalyzes the hydrolytic cleavage of argininosuccinic acid (ASA) to fumarate and arginine, an essential step of the urea cycle which is crucial for the detoxification of ammonia. This reaction is also involved in the biosynthesis of arginine. In addition, ASL shares high sequence homology with the avian and reptilian eye lens protein, d-crystallin. Mutations in the gene encoding ASL leads to an accumulation of ASA in body fluids and results in Arginosuc-cinic aciduria (ASAuria), an autosomal recessive disorder that is characterized by hyperammonemia, liver enlargement, convulsions, physical and mental retardation, episodic unconsciousness and dry and brittle hair showing trich-orrhexis nodosa (weak points or nodes in the hair shaft).
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Intragenic complementation and the structure and function of argininosuccinate lyase. | Yu, B. and Howell, PL. 2000. Cell Mol Life Sci. 57: 1637-51. PMID: 11092456
- Three-dimensional structure of the argininosuccinate lyase frequently complementing allele Q286R. | Sampaleanu, LM., et al. 2001. Biochemistry. 40: 15570-80. PMID: 11747432
- Mechanisms for intragenic complementation at the human argininosuccinate lyase locus. | Yu, B., et al. 2001. Biochemistry. 40: 15581-90. PMID: 11747433
- Argininosuccinate lyase (ASL) deficiency: mutation analysis in 27 patients and a completed structure of the human ASL gene. | Linnebank, M., et al. 2002. Hum Genet. 111: 350-9. PMID: 12384776
- A novel stop codon mutation (X465Y) in the argininosuccinate lyase gene in a patient with argininosuccinic aciduria. | Tanaka, T., et al. 2002. Tohoku J Exp Med. 198: 119-24. PMID: 12512996
- Critical role of tryptophanyl residues in the conformational stability of goose delta-crystallin. | Lee, HJ., et al. 2006. Exp Eye Res. 83: 658-66. PMID: 16677632
- Deletion hotspot in the argininosuccinate lyase gene: association with topoisomerase II and DNA polymerase alpha sites. | Christodoulou, J., et al. 2006. Hum Mutat. 27: 1065-71. PMID: 16941645
- Argininosuccinate lyase deficiency: mutational spectrum in Italian patients and identification of a novel ASL pseudogene. | Trevisson, E., et al. 2007. Hum Mutat. 28: 694-702. PMID: 17326097
- Human argininosuccinate lyase: a structural basis for intragenic complementation. | Turner, MA., et al. 1997. Proc Natl Acad Sci U S A. 94: 9063-8. PMID: 9256435
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASL Antibody (E-5) | sc-374353 | 200 µg/ml | $310.00 | |||
ASL Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-529726 | 200 µg Ab; 10 µg BP | $348.00 | |||
ASL Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-522709 | 200 µg Ab, 40 µg BP | $348.00 | |||
ASL Antibody (E-5): m-IgG1 BP-HRP Bundle | sc-543654 | 200 µg Ab; 20 µg BP | $348.00 | |||
ASL Antibody (E-5) AC | sc-374353 AC | 500 µg/ml, 25% agarose | $408.00 | |||
ASL Antibody (E-5) HRP | sc-374353 HRP | 200 µg/ml | $310.00 | |||
ASL Antibody (E-5) FITC | sc-374353 FITC | 200 µg/ml | $324.00 | |||
ASL Antibody (E-5) PE | sc-374353 PE | 200 µg/ml | $337.00 | |||
ASL Antibody (E-5) Alexa Fluor® 488 | sc-374353 AF488 | 200 µg/ml | $350.00 | |||
ASL Antibody (E-5) Alexa Fluor® 546 | sc-374353 AF546 | 200 µg/ml | $350.00 | |||
ASL Antibody (E-5) Alexa Fluor® 594 | sc-374353 AF594 | 200 µg/ml | $350.00 | |||
ASL Antibody (E-5) Alexa Fluor® 647 | sc-374353 AF647 | 200 µg/ml | $350.00 | |||
ASL Antibody (E-5) Alexa Fluor® 680 | sc-374353 AF680 | 200 µg/ml | $350.00 | |||
ASL Antibody (E-5) Alexa Fluor® 790 | sc-374353 AF790 | 200 µg/ml | $350.00 | |||
ASL (E-5) Neutralizing Peptide | sc-374353 P | 100 µg/0.5 ml | $67.00 |