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CPS1 Antibody (B-1): sc-376190
- CPS1 Antibody (B-1) is a mouse monoclonal IgG1 κ CPS1 antibody, cited in 7 publications, provided at 200 µg/ml
- raised against amino acids 1361-1500 mapping at the C-terminus of CPS1 of human origin
- CPS1 Antibody (B-1) is recommended for detection of CPS1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CPS1 Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CPS1 (B-1): sc-376190.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CPS1 Antibody (B-1) for WB and IHC(P) applications. These reagents are now offered in bundles with CPS1 Antibody (B-1) (see ordering information below).
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SEE ALSO...
CPS1 Antibody (B-1) is an IgG1 κ mouse monoclonal CPS1 antibody (also designated CPS1 antibody) that detects the CPS1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. CPS1 Antibody (B-1) is available as both the non-conjugated anti-CPS1 antibody form, as well as multiple conjugated forms of anti-CPS1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The multicomplex protein, carbamoyl-phosphate synthetase-aspartate carbamoyl transferase-dihydro-orotase (CAD), consists of three distinct proteins, carbamoyl phosphate synthetase 2 (CPS2), aspartate transcarbamylase, and dihydro-orotase, which catalyze the second and third steps of pyrimidine biosynthesis. CAD is allosterically regulated by the phosphorylation of CPS2 by cyclic AMP-dependent protein kinase, and this activation enables CPS2 to catalyze the rate-limiting step of pyrimidine synthesis. CAD is expressed in brain and skeletal muscle. A related protein, carbamoyl phosphate synthetase 1 (CPS1) is expressed in liver. CPS1 catalyzes the rate-limiting step in the urea cycle, and deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Functional linkage between the glutaminase and synthetase domains of carbamoyl-phosphate synthetase. Role of serine 44 in carbamoyl-phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (cad). | Hewagama, A., et al. 1999. J Biol Chem. 274: 28240-5. PMID: 10497179
- Regulation of carbamoyl phosphate synthetase by MAP kinase. | Graves, LM., et al. 2000. Nature. 403: 328-32. PMID: 10659854
- Localization of the multifunctional protein CAD in astrocytes of rodent brain. | Cammer, W. and Downing, M. 1991. J Histochem Cytochem. 39: 695-700. PMID: 1673139
- Cloning and sequence of a cDNA encoding human carbamyl phosphate synthetase I: molecular analysis of hyperammonemia. | Haraguchi, Y., et al. 1991. Gene. 107: 335-40. PMID: 1840546
- Phosphorylation and activation of hamster carbamyl phosphate synthetase II by cAMP-dependent protein kinase. A novel mechanism for regulation of pyrimidine nucleotide biosynthesis. | Carrey, EA., et al. 1985. EMBO J. 4: 3735-42. PMID: 4092695
- Phosphorylation and dephosphorylation of carbamoyl-phosphate synthetase II complex of rat ascites hepatoma cells. | Otsuki, T., et al. 1981. J Biochem. 89: 1367-74. PMID: 6115855
- Mice deficient in the urea-cycle enzyme, carbamoyl phosphate synthetase I, die during the early neonatal period from hyperammonemia. | Schofield, JP., et al. 1999. Hepatology. 29: 181-5. PMID: 9862865
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPS1 Antibody (B-1) | sc-376190 | 200 µg/ml | $310.00 | |||
CPS1 Antibody (B-1): m-IgG Fc BP-HRP Bundle | sc-529835 | 200 µg Ab; 10 µg BP | $348.00 | |||
CPS1 Antibody (B-1): m-IgGκ BP-HRP Bundle | sc-522870 | 200 µg Ab, 40 µg BP | $348.00 | |||
CPS1 Antibody (B-1): m-IgG1 BP-HRP Bundle | sc-543714 | 200 µg Ab; 20 µg BP | $348.00 | |||
CPS1 Antibody (B-1) AC | sc-376190 AC | 500 µg/ml, 25% agarose | $408.00 | |||
CPS1 Antibody (B-1) HRP | sc-376190 HRP | 200 µg/ml | $310.00 | |||
CPS1 Antibody (B-1) FITC | sc-376190 FITC | 200 µg/ml | $324.00 | |||
CPS1 Antibody (B-1) PE | sc-376190 PE | 200 µg/ml | $337.00 | |||
CPS1 Antibody (B-1) Alexa Fluor® 488 | sc-376190 AF488 | 200 µg/ml | $350.00 | |||
CPS1 Antibody (B-1) Alexa Fluor® 546 | sc-376190 AF546 | 200 µg/ml | $350.00 | |||
CPS1 Antibody (B-1) Alexa Fluor® 594 | sc-376190 AF594 | 200 µg/ml | $350.00 | |||
CPS1 Antibody (B-1) Alexa Fluor® 647 | sc-376190 AF647 | 200 µg/ml | $350.00 | |||
CPS1 Antibody (B-1) Alexa Fluor® 680 | sc-376190 AF680 | 200 µg/ml | $350.00 | |||
CPS1 Antibody (B-1) Alexa Fluor® 790 | sc-376190 AF790 | 200 µg/ml | $350.00 |