CTH Antibody (F-1): sc-374249

CTH Antibody (F-1) is an IgG₁ κ mouse monoclonal CTH antibody (also designated Cystathionine gamma-lyase antibody, CTH antibody, cytoplasmic cystathione methionine cysteine trans-sulfuration pathway related antibody, or CSE antibody) that detects the CTH protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. CTH Antibody (F-1) is available as both the non-conjugated anti-CTH antibody form, as well as multiple conjugated forms of anti-CTH antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. CTH (cystathionine γ-lyase), also known as CSE or γ-cystathionase, is a member of the trans-sulfuration enzyme family and participates in the trans-sulfuration pathway. CTH is a cytoplasmic enzyme produced in the cytosol and is responsible for catalyzing the pyridoxal phosphate-dependent β-disulfide elimination reaction resulting in ammonium, pyruvate and thiocysteine. The thiocysteine that is produced may then react with other thiols (or cysteine) and form hydrogen sulfide (H2S). Thus, CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion.

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References:

1. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase.  |  Lu, Y., et al. 1992. Biochem Biophys Res Commun. 189: 749-58. PMID: 1339280
2. Murine cystathionine gamma-lyase: complete cDNA and genomic sequences, promoter activity, tissue distribution and developmental expression.  |  Ishii, I., et al. 2004. Biochem J. 381: 113-23. PMID: 15038791
3. Endogenous production of hydrogen sulfide in mammals.  |  Kamoun, P. 2004. Amino Acids. 26: 243-54. PMID: 15221504
4. [H2S, a new neuromodulator].  |  Kamoun, P. 2004. Med Sci (Paris). 20: 697-700. PMID: 15329822
5. Cystathionine gamma-lyase overexpression inhibits cell proliferation via a H2S-dependent modulation of ERK1/2 phosphorylation and p21Cip/WAK-1.  |  Yang, G., et al. 2004. J Biol Chem. 279: 49199-205. PMID: 15347670
6. New roles for cysteine and transsulfuration enzymes: production of H2S, a neuromodulator and smooth muscle relaxant.  |  Dominy, JE. and Stipanuk, MH. 2004. Nutr Rev. 62: 348-53. PMID: 15497768
7. Pro-apoptotic effect of endogenous H2S on human aorta smooth muscle cells.  |  Yang, G., et al. 2006. FASEB J. 20: 553-5. PMID: 16507767
8. Hydrogen sulfide is a novel prosecretory neuromodulator in the Guinea-pig and human colon.  |  Schicho, R., et al. 2006. Gastroenterology. 131: 1542-52. PMID: 17101327
9. Contractile and vasorelaxant effects of hydrogen sulfide and its biosynthesis in the human internal mammary artery.  |  Webb, GD., et al. 2008. J Pharmacol Exp Ther. 324: 876-82. PMID: 18029544

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CTH Antibody (F-1) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-CTH Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CTH Antibody (F-1) Specifications

Alternate Product Name

Cystathionase

Product Clone Name

F-1

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

43-75

Epitope Target

CTH

Epitope Species

human

Product Reactivity/Detection

CTH isoforms 1 and 2

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

bovine and porcine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

27 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin