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DAAO Antibody (B-3): sc-398757
- DAAO Antibody (B-3) is a mouse monoclonal IgG2a κ DAAO antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 312-337 near the C-terminus of DAAO of mouse origin
- DAAO Antibody (B-3) is recommended for detection of DAAO of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DAAO Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DAAO (B-3): sc-398757.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DAAO Antibody (B-3) for WB and IHC(P) applications. These reagents are now offered in bundles with DAAO Antibody (B-3) (see ordering information below).
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SEE ALSO...
DAAO Antibody (B-3) is an IgG2a κ mouse monoclonal DAAO antibody (also designated DAAO antibody) that detects the DAAO protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. DAAO Antibody (B-3) is available as both the non-conjugated anti-DAAO antibody form, as well as multiple conjugated forms of anti-DAAO antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. D-amino acid oxidase (DAAO) metabolizes exogenous D-amino acids that accumulate during aging, and may modulate the level of D-serine in the brain, acting as a detoxifying agent. DAAO is specific for the D-configuration of amino acids and exhibits a preference for those with small, hydrophobic side chains and polar, aromatic and basic functional groups. Single base-pair substitutions at certain amino acid residues of the DAAO enzyme result in the loss of DAO activity in mice. Mutation of the enzyme at residue Y238, one of the few conserved residues in the active site of DAAO, leads to a significantly slower rate of product release relative to the wild-type enzyme, indicating the importance of this residue in controlling access to the active site. In addition, the other conserved residues in the active site of DAAO do not play a role in acid-base catalysis but rather are involved in substrate interactions, which along with the mode of substrate orientation into the active site, suggest a hybrid transfer type of mechanism for catalysis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- D-Amino acid oxidase: new findings. | Pilone, MS. 2000. Cell Mol Life Sci. 57: 1732-47. PMID: 11130179
- D-amino acid oxidase: its potential in the production of 7-aminocephalosporanic acid. | Mujawar, SK. 1999. Hindustan Antibiot Bull. 41: 1-14. PMID: 12024974
- Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study. | Boselli, A., et al. 2002. Eur J Biochem. 269: 4762-71. PMID: 12354107
- A single-base-pair substitution abolishes D-amino-acid oxidase activity in the mouse. | Sasaki, M., et al. 1992. Biochim Biophys Acta. 1139: 315-8. PMID: 1355365
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAAO Antibody (B-3) | sc-398757 | 200 µg/ml | $310.00 | |||
DAAO Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-530919 | 200 µg Ab; 10 µg BP | $348.00 | |||
DAAO Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-524473 | 200 µg Ab, 40 µg BP | $348.00 | |||
DAAO Antibody (B-3): m-IgG2a BP-HRP Bundle | sc-547631 | 200 µg Ab; 10 µg BP | $348.00 | |||
DAAO Antibody (B-3) AC | sc-398757 AC | 500 µg/ml, 25% agarose | $408.00 | |||
DAAO Antibody (B-3) HRP | sc-398757 HRP | 200 µg/ml | $310.00 | |||
DAAO Antibody (B-3) FITC | sc-398757 FITC | 200 µg/ml | $324.00 | |||
DAAO Antibody (B-3) PE | sc-398757 PE | 200 µg/ml | $337.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 488 | sc-398757 AF488 | 200 µg/ml | $350.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 546 | sc-398757 AF546 | 200 µg/ml | $350.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 594 | sc-398757 AF594 | 200 µg/ml | $350.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 647 | sc-398757 AF647 | 200 µg/ml | $350.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 680 | sc-398757 AF680 | 200 µg/ml | $350.00 | |||
DAAO Antibody (B-3) Alexa Fluor® 790 | sc-398757 AF790 | 200 µg/ml | $350.00 | |||
DAAO (B-3) Neutralizing Peptide | sc-398757 P | 100 µg/0.5 ml | $67.00 |