Click on image or enlarge button to enlarge
GFAT1 Antibody (D-9): sc-377479
- GFAT1 Antibody (D-9) is a mouse monoclonal IgG1 κ GFAT1 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 226-274 mapping within an internal region of GFAT1 of human origin
- GFAT1 Antibody (D-9) is recommended for detection of GFAT1 of human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and porcine
- Anti-GFAT1 Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GFAT1 (D-9): sc-377479.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GFAT1 Antibody (D-9) for WB applications. These reagents are now offered in bundles with GFAT1 Antibody (D-9) (see ordering information below).
QUICK LINKS
GFAT1 Antibody (D-9) is an IgG1 κ mouse monoclonal GFAT1 antibody (also designated GFAT1 antibody) that detects the GFAT1 protein of human origin by WB, IP, IF and ELISA. GFAT1 Antibody (D-9) is available as both the non-conjugated anti-GFAT1 antibody form, as well as multiple conjugated forms of anti-GFAT1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Glutamine:fructose-6-phosphate amidotransferase (GFAT1) is the first and rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT1, a member of the N-terminal nucleophile class of amidotransferases, converts fructose-6-phosphate into N-acetylglucosamine-6-phosphate. Hyperglycemia-induced insulin resistance, a condition in which exposure to high concentrations of glucose and insulin results in insulin resistance, may result from increased glucose metabolism through the HBP. Hypergylcemia-induced insulin resistance is a characteristic feature of type 2 diabetes. Consequently, GFAT1 is a potential therapeutic target in the treatment of type 2 diabetes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Activation of the hexosamine pathway leads to deterioration of pancreatic beta-cell function through the induction of oxidative stress. | Kaneto, H., et al. 2001. J Biol Chem. 276: 31099-104. PMID: 11390407
- Identification of GFAT1-L, a novel splice variant of human glutamine: fructose-6-phosphate amidotransferase (GFAT1) that is expressed abundantly in skeletal muscle. | Niimi, M., et al. 2001. J Hum Genet. 46: 566-71. PMID: 11587069
- A novel variant of glutamine: fructose-6-phosphate amidotransferase-1 (GFAT1) mRNA is selectively expressed in striated muscle. | DeHaven, JE., et al. 2001. Diabetes. 50: 2419-24. PMID: 11679416
- Kinetic characterization of human glutamine-fructose-6-phosphate amidotransferase I: potent feedback inhibition by glucosamine 6-phosphate. | Broschat, KO., et al. 2002. J Biol Chem. 277: 14764-70. PMID: 11842094
- A radiometric assay for glutamine:fructose-6-phosphate amidotransferase. | Broschat, KO., et al. 2002. Anal Biochem. 305: 10-5. PMID: 12018941
- Molecular screening of the human glutamine-fructose-6-phosphate amidotransferase 1 (GFPT1) gene and association studies with diabetes and diabetic nephropathy. | Elbein, SC., et al. 2004. Mol Genet Metab. 82: 321-8. PMID: 15308130
- Molecular therapeutic target for type-2 diabetes. | Chou, KC. 2004. J Proteome Res. 3: 1284-8. PMID: 15595739
- Partial characterisation of the human GFAT promoter: effect of single nucleotide polymorphisms on promoter function. | Burt, D., et al. 2005. Biochim Biophys Acta. 1740: 85-90. PMID: 15878746
- Expression of glutamine:fructose-6-phosphate amidotransferase in human tissues: evidence for high variability and distinct regulation in diabetes. | Nerlich, AG., et al. 1998. Diabetes. 47: 170-8. PMID: 9519709
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GFAT1 Antibody (D-9) | sc-377479 | 200 µg/ml | $310.00 | |||
GFAT1 Antibody (D-9): m-IgG Fc BP-HRP Bundle | sc-530028 | 200 µg Ab; 10 µg BP | $348.00 | |||
GFAT1 Antibody (D-9): m-IgGκ BP-HRP Bundle | sc-523343 | 200 µg Ab, 40 µg BP | $348.00 | |||
GFAT1 Antibody (D-9): m-IgG1 BP-HRP Bundle | sc-543827 | 200 µg Ab; 20 µg BP | $348.00 | |||
GFAT1 Antibody (D-9) AC | sc-377479 AC | 500 µg/ml, 25% agarose | $408.00 | |||
GFAT1 Antibody (D-9) HRP | sc-377479 HRP | 200 µg/ml | $310.00 | |||
GFAT1 Antibody (D-9) FITC | sc-377479 FITC | 200 µg/ml | $324.00 | |||
GFAT1 Antibody (D-9) PE | sc-377479 PE | 200 µg/ml | $337.00 | |||
GFAT1 Antibody (D-9) Alexa Fluor® 488 | sc-377479 AF488 | 200 µg/ml | $350.00 | |||
GFAT1 Antibody (D-9) Alexa Fluor® 546 | sc-377479 AF546 | 200 µg/ml | $350.00 | |||
GFAT1 Antibody (D-9) Alexa Fluor® 594 | sc-377479 AF594 | 200 µg/ml | $350.00 | |||
GFAT1 Antibody (D-9) Alexa Fluor® 647 | sc-377479 AF647 | 200 µg/ml | $350.00 | |||
GFAT1 Antibody (D-9) Alexa Fluor® 680 | sc-377479 AF680 | 200 µg/ml | $350.00 | |||
GFAT1 Antibody (D-9) Alexa Fluor® 790 | sc-377479 AF790 | 200 µg/ml | $350.00 |