Heme Oxygenase 2/3 Antibody (A-3): sc-166342

Heme Oxygenase 2/3 Antibody (A-3) is an IgG₁ κ mouse monoclonal Heme Oxygenase 2/3 antibody (also designated HO-2/3 antibody or HMOX2 antibody) that detects the Heme Oxygenase 2/3 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Heme Oxygenase 2/3 Antibody (A-3) is available as both the non-conjugated anti-Heme Oxygenase 2/3 antibody form, as well as multiple conjugated forms of anti-Heme Oxygenase 2/3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Developmental biology of heme oxygenase.  |  Rodgers, PA. and Stevenson, DK. 1990. Clin Perinatol. 17: 275-91. PMID: 2196131
2. Heme oxygenase: function, multiplicity, regulatory mechanisms, and clinical applications.  |  Maines, MD. 1988. FASEB J. 2: 2557-68. PMID: 3290025
3. Isolation and characterization of the mouse heme oxygenase-1 gene. Distal 5′ sequences are required for induction by heme or heavy metals.  |  Alam, J., et al. 1994. J Biol Chem. 269: 1001-9. PMID: 8288554
4. The heme oxygenase system: a regulator of second messenger gases.  |  Maines, MD. 1997. Annu Rev Pharmacol Toxicol. 37: 517-54. PMID: 9131263
5. Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heme oxygenase-3.  |  McCoubrey, WK., et al. 1997. Eur J Biochem. 247: 725-32. PMID: 9266719
6. Nitric oxide and carbon monoxide: parallel roles as neural messengers.  |  Snyder, SH., et al. 1998. Brain Res Brain Res Rev. 26: 167-75. PMID: 9651518
7. Heme oxygenase-1-derived carbon monoxide contributes to the suppression of acute hypertensive responses in vivo.  |  Motterlini, R., et al. 1998. Circ Res. 83: 568-77. PMID: 9734480

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Heme Oxygenase 2/3 Antibody (A-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Heme Oxygenase 2/3 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Heme Oxygenase 2/3 Antibody (A-3) Specifications

Alternate Product Name

Heme Oxygenase 2/3

Product Clone Name

A-3

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

1-300

Epitope Target

Heme Oxygenase 2

Epitope Species

mouse

Product Reactivity/Detection

Heme Oxygenase 2 of mouse, rat and human origin, and Heme Oxygenase 3 of mouse origin

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

0 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Heme Oxygenase 1/HMOX1 antibody is Heme Oxygenase 1/HMOX1 Antibody (A-3): sc-136960.