IDH2 Antibody (B-6): sc-374476

IDH2 Antibody (B-6) is an IgG1 κ mouse monoclonal IDH2 antibody that detects IDH2 of human origin by WB, IP, IF and ELISA. IDH2 Antibody (B-6) is available as the non-conjugated anti-IDH2 antibody form, as well as multiple conjugated forms of anti-IDH2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. IDH2 (isocitrate dehydrogenase 2 (NADP+), mitochondrial), also designated NADP+-specific ICDH; isocitrate dehydrogenase, mitochondrial; and oxalosuccinate decarboxylase, is a 452 amino acid enzyme encoded by the human gene IDH2. IDH2 belongs to the isocitrate and isopropylmalate dehydrogenases family and contains two nucleotide binding regions. IDH2 is involved in the reduction of NADP+ to NADPH and maintains the supply of glutathione (GSH) in mitochondria. It is believed to play a role in intermediary metabolism and energy production. IDH2 also tightly associates with the pyruvate dehydrogenase complex. IDH2 is found in the mitochondrion as a homodimer and can bind one magnesium or manganese ion per subunit.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Isolation of a Histoplasma capsulatum cDNA that complements a mitochondrial NAD(+)-isocitrate dehydrogenase subunit I-deficient mutant of Saccharomyces cerevisiae.  |  Johnson, CH. and McEwen, JE. 1999. Yeast. 15: 799-804. PMID: 10398348
2. Effect of NAD+-dependent isocitrate dehydrogenase gene (IDH1, IDH2) disruption of sake yeast on organic acid composition in sake mash.  |  Asano, T., et al. 1999. J Biosci Bioeng. 88: 258-63. PMID: 16232608
3. Mitochondrial isocitrate dehydrogenase protects human neuroblastoma SH-SY5Y cells against oxidative stress.  |  Kim, SJ., et al. 2007. J Neurosci Res. 85: 139-52. PMID: 17075901
4. Assignment of cytoplasmic alpha-mannosidase (MANA) and confirmation of mitochondrial isocitrate dehydrogenase (IDHM) to the q11 leads to qter region of chromosome 15 in man.  |  Champion, MJ., et al. 1978. Cytogenet Cell Genet. 22: 498-502. PMID: 752528
5. Expression of human mitochondrial NADP-dependent isocitrate dehydrogenase during lymphocyte activation.  |  Luo, H., et al. 1996. J Cell Biochem. 60: 495-507. PMID: 8707889
6. Assignment of the human mitochondrial NAD+ -specific isocitrate dehydrogenase alpha subunit (IDH3A) gene to 15q25.1-->q25.2by in situ hybridization.  |  Huh, TL., et al. 1996. Genomics. 32: 295-6. PMID: 8833160
7. Assignment of the human mitochondrial NADP(+)-specific isocitrate dehydrogenase (IDH2) gene to 15q26.1 by in situ hybridization.  |  Oh, IU., et al. 1996. Genomics. 38: 104-6. PMID: 8954790
8. Assignment of a gene for human mitochondrial isocitrate dehydrogenase (ICD-M, EC 1.1.1.41) to chromosome 15.  |  Grzeschik, KH. 1976. Hum Genet. 34: 23-8. PMID: 965003
9. Molecular characterization of higher plant NAD-dependent isocitrate dehydrogenase: evidence for a heteromeric structure by the complementation of yeast mutants.  |  Lancien, M., et al. 1998. Plant J. 16: 325-33. PMID: 9881153

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IDH2 Antibody (B-6) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-IDH2 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IDH2 Antibody (B-6) Specifications

Alternate Product Name

IDH2

Product Clone Name

B-6

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

71-110

Epitope Target

IDH2

Epitope Species

human

Product Reactivity/Detection

IDH2

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

8 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred IDH1/2 antibody is IDH1/2 Antibody (G-11): sc-373816.