Rho G Antibody (1F3 B3 E5): sc-80015

Rho G Antibody (1F3 B3 E5) is an IgG_2a κ mouse monoclonal Rho G antibody (also designated Rho G antibody) that detects the Rho G protein of mouse, rat and human origin by WB and IP. Rho G Antibody (1F3 B3 E5) is available as both the non-conjugated anti-Rho G antibody form, as well as multiple conjugated forms of anti-Rho G antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Post-translational modifications of p21rho proteins.  |  Adamson, P., et al. 1992. J Biol Chem. 267: 20033-8. PMID: 1400319
2. Expression of the ras-related ralA, rho12 and rab genes in adult mouse tissues.  |  Olofsson, B., et al. 1988. Oncogene. 3: 231-4. PMID: 2457862
3. Identification of distinct cytoplasmic targets for ras/R-ras and rho regulatory proteins.  |  Garrett, MD., et al. 1989. J Biol Chem. 264: 10-3. PMID: 2491843
4. Scrape-loading of Swiss 3T3 cells with ras protein rapidly activates protein kinase C in the absence of phosphoinositide hydrolysis.  |  Morris, JD., et al. 1989. Oncogene. 4: 27-31. PMID: 2536916
5. The ras superfamily proteins.  |  Chardin, P. 1988. Biochimie. 70: 865-8. PMID: 3145021
6. ras genes.  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
7. Nucleotide sequence of human rho cDNA clone 12.  |  Yeramian, P., et al. 1987. Nucleic Acids Res. 15: 1869. PMID: 3822842
8. A novel ras-related gene family.  |  Madaule, P. and Axel, R. 1985. Cell. 41: 31-40. PMID: 3888408

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rho G Antibody (1F3 B3 E5) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-Rho G Antibody (1F3 B3 E5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rho G Antibody (1F3 B3 E5) Specifications

Alternate Product Name

Rho G

Product Clone Name

1F3 B3 E5

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

Epitope Target

Rho G

Epitope Species

mouse

Product Reactivity/Detection

endogenous Rho G

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

11 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Kappa Light Chain Binding Proteins

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin