Aquaporin 2/AQP2 Antibody (E13-21): sc-47710

Aquaporin 2/AQP2 Antibody (E13-21) is an IgM κ mouse monoclonal AQP2 antibody that detects AQP2 of mouse, rat and human origin by WB and IF. Aquaporin 2/AQP2 Antibody (E13-21) is available as both conjugated and non-conjugated anti-AQP2 antibody forms. Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation, and the secretion and reabsorption of cerebrospinal fluid and aqueous humor.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. The mammalian aquaporin water channel family: A promising new drug target.  |  Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
2. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family.  |  Preston, GM. and Agre, P. 1991. Proc Natl Acad Sci U S A. 88: 11110-4. PMID: 1722319
3. Cloning, characterization, and chromosomal mapping of human aquaporin of collecting duct.  |  Sasaki, S., et al. 1994. J Clin Invest. 93: 1250-6. PMID: 7510718
4. Structure and chromosomal localization of a human water channel (AQP3) gene.  |  Ishibashi, K., et al. 1995. Genomics. 27: 352-4. PMID: 7558005
5. cDNA cloning, gene organization, and chromosomal localization of a human mercurial insensitive water channel. Evidence for distinct transcriptional units.  |  Yang, B., et al. 1995. J Biol Chem. 270: 22907-13. PMID: 7559426
6. Requirement of human renal water channel aquaporin-2 for vasopressin-dependent concentration of urine.  |  Deen, PM., et al. 1994. Science. 264: 92-5. PMID: 8140421
7. The human Aquaporin-5 gene. Molecular characterization and chromosomal localization.  |  Lee, MD., et al. 1996. J Biol Chem. 271: 8599-604. PMID: 8621489
8. Expression and functional analysis of water channels in a stably AQP2-transfected human collecting duct cell line.  |  Valenti, G., et al. 1996. J Biol Chem. 271: 24365-70. PMID: 8798691
9. Aquaporins.  |  Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Aquaporin 2/AQP2 Antibody (E13-21) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Aquaporin 2/AQP2 Antibody (E13-21) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Aquaporin 2/AQP2 Antibody (E13-21) Specifications

Alternate Product Name

aquaporin 2

Product Clone Name

E13-21

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgM kappa light chain

Amino Acids

Epitope Target

AQP2

Epitope Species

rat

Product Reactivity/Detection

AQP2

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF

Vialing Information

Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

4 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Kappa Light Chain Binding Proteins

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790