Aquaporin 4/AQP4 Antibody (4/18): sc-32739

AQP4 Antibody (4/18) is an IgG₃ κ mouse monoclonal AQP4 antibody (also designated aquaporin 4 antibody) that detects the AQP4 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. AQP4 Antibody (4/18) is available as both the non-conjugated anti-AQP4 antibody form, as well as multiple conjugated forms of anti-AQP4 antibody, including agarose, PE, FITC and multiple Alexa Fluor^® conjugates. In skeletal muscle, AQP4 (Aquaporin 4, also known as mercurial insensitive water channel), localizes to the sarcolemma of fast-twitch muscle fibers. Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor.

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References:

1. The mammalian aquaporin water channel family: A promising new drug target.  |  Beitz, E. and Schultz, JE. 1999. Curr Med Chem. 6: 457-67. PMID: 10213793
2. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family.  |  Preston, GM. and Agre, P. 1991. Proc Natl Acad Sci U S A. 88: 11110-4. PMID: 1722319
3. Structure and chromosomal localization of a human water channel (AQP3) gene.  |  Ishibashi, K., et al. 1995. Genomics. 27: 352-4. PMID: 7558005
4. cDNA cloning, gene organization, and chromosomal localization of a human mercurial insensitive water channel. Evidence for distinct transcriptional units.  |  Yang, B., et al. 1995. J Biol Chem. 270: 22907-13. PMID: 7559426
5. Requirement of human renal water channel aquaporin-2 for vasopressin-dependent concentration of urine.  |  Deen, PM., et al. 1994. Science. 264: 92-5. PMID: 8140421
6. The human Aquaporin-5 gene. Molecular characterization and chromosomal localization.  |  Lee, MD., et al. 1996. J Biol Chem. 271: 8599-604. PMID: 8621489
7. Cloning and chromosomal localization of mouse aquaporin 4: exclusion of a candidate mutant phenotype, ataxia.  |  Turtzo, LC., et al. 1997. Genomics. 41: 267-70. PMID: 9143504
8. Expression of aquaporin-4 in fast-twitch fibers of mammalian skeletal muscle.  |  Frigeri, A., et al. 1998. J Clin Invest. 102: 695-703. PMID: 9710437
9. Aquaporins.  |  Echevarría, M. and Ilundáin, AA. 1998. J Physiol Biochem. 54: 107-18. PMID: 9858131

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Aquaporin 4/AQP4 Antibody (4/18) has 39 citations in a variety of scientific publications.

Primary antibodies like Anti-Aquaporin 4/AQP4 Antibody (4/18) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Aquaporin 4/AQP4 Antibody (4/18) Specifications

Alternate Product Name

aquaporin 4

Product Clone Name

4/18

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₃ kappa light chain

Amino Acids

301-318

Epitope Target

AQP4

Epitope Species

human

Product Reactivity/Detection

AQP4

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), FCM

Vialing Information

Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

39 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, PE: Phycoerythrin