Click on image or enlarge button to enlarge
ATP5B Antibody (E-1): sc-55597
- ATP5B Antibody (E-1) is a mouse monoclonal IgG1 κ ATP5B antibody, cited in 16 publications, provided at 200 µg/ml
- raised against amino acids 230-529 mapping at the C-terminus of ATP5B of human origin
- ATP5B Antibody (E-1) is recommended for detection of ATP5B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ATP5B Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ATP5B (E-1): sc-55597.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ATP5B Antibody (E-1) for WB and IHC(P) applications. These reagents are now offered in bundles with ATP5B Antibody (E-1) (see ordering information below).
QUICK LINKS
ATP5B Antibody (E-1) is an IgG1 κ mouse monoclonal ATP5B antibody (also designated ATP5F1B antibody, ATPB antibody, ATP Synthase F1 Subunit Beta antibody, ATP Synthase Subunit Beta Mitochondrial antibody, ATP5B antibody, ATPMB antibody, Mitochondrial ATP Synthetase Beta Subunit antibody, or Mitochondrial ATP Synthase Beta Subunit antibody) that detects the ATP5B protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. ATP5B Antibody (E-1) is available as both the non-conjugated anti-ATP5B antibody form, as well as multiple conjugated forms of anti-ATP5B antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Mitochondrial ATP synthase is composed of two multi-subunit complexes that utilize an inner membrane electrochemical gradient to catalyze the synthesis of ATP during oxidative phosphorylation. The two multi-subunit complexes are designated F1 and F0, the former of which comprises the soluble catalytic core and the latter of which comprises the membrane-spanning proton channel of ATP synthase. F1 consists of five distinct subunits, designated ATP5A, ATP5B, ATP5C1, ATP5D and ATP5E, while F0 consists of ten subunits, designated ATP5H, ATP5G1, ATP5I, ATP5G2, ATP5J2, ATP5J, ATP5G3, ATP5S, ATP5F1 and ATP5L. ATP5B, also designated ATPMB, ATPSB or mitochondrial ATP synthetase, beta subunit, is a 529 amino acid protein that localizes to the mitochondrial membrane and exists as a subunit of the F0 complex. ATP5B is encoded by a nuclear gene and assembled with the other subunits encoded by both mitochondrial and nuclear genes. The ATP5B gene is activated by members of the Ets family of transcription factors, suggesting that Ets transcription factors are involved in the enhanced expression of the ATP5B gene in highly proliferating cells and in the coordinate transcription of nuclear genes for mitochondrial proteins. ATP5B mRNA levels vary among species through transcriptional control with high expression levels in heart, lower levels in skeletal muscle and the lowest levels in liver and kidney.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Understanding ATP synthesis: structure and mechanism of the F1-ATPase (Review). | Leyva, JA., et al. 2003. Mol Membr Biol. 20: 27-33. PMID: 12745923
- Kinetic studies of ATP synthase: the case for the positional change mechanism. | LaNoue, KF. and Duszynski, J. 1992. J Bioenerg Biomembr. 24: 499-506. PMID: 1429543
- Regulation of the mitochondrial ATPase in situ in cardiac muscle: role of the inhibitor subunit. | Rouslin, W. 1991. J Bioenerg Biomembr. 23: 873-88. PMID: 1838111
- The human ATP synthase beta subunit gene: sequence analysis, chromosome assignment, and differential expression. | Neckelmann, N., et al. 1989. Genomics. 5: 829-43. PMID: 2687158
- Human F1-ATPase: molecular cloning of cDNA for the beta subunit. | Ohta, S. and Kagawa, Y. 1986. J Biochem. 99: 135-41. PMID: 2870059
- ETS transcription factors regulate the expression of the gene for the human mitochondrial ATP synthase beta-subunit. | Villena, JA., et al. 1994. J Biol Chem. 269: 32649-54. PMID: 7798271
- Energy transduction in ATP synthase. | Elston, T., et al. 1998. Nature. 391: 510-3. PMID: 9461222
- Energy transduction in the F1 motor of ATP synthase. | Wang, H. and Oster, G. 1998. Nature. 396: 279-82. PMID: 9834036
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP5B Antibody (E-1) | sc-55597 | 200 µg/ml | $310.00 | |||
ATP5B Antibody (E-1): m-IgG Fc BP-HRP Bundle | sc-528608 | 200 µg Ab; 10 µg BP | $348.00 | |||
ATP5B Antibody (E-1): m-IgGκ BP-HRP Bundle | sc-521028 | 200 µg Ab, 40 µg BP | $348.00 | |||
ATP5B Antibody (E-1): m-IgG1 BP-HRP Bundle | sc-543026 | 200 µg Ab; 20 µg BP | $348.00 | |||
ATP5B Antibody (E-1) AC | sc-55597 AC | 500 µg/ml, 25% agarose | $408.00 | |||
ATP5B Antibody (E-1) HRP | sc-55597 HRP | 200 µg/ml | $310.00 | |||
ATP5B Antibody (E-1) FITC | sc-55597 FITC | 200 µg/ml | $324.00 | |||
ATP5B Antibody (E-1) PE | sc-55597 PE | 200 µg/ml | $337.00 | |||
ATP5B Antibody (E-1) Alexa Fluor® 488 | sc-55597 AF488 | 200 µg/ml | $350.00 | |||
ATP5B Antibody (E-1) Alexa Fluor® 546 | sc-55597 AF546 | 200 µg/ml | $350.00 | |||
ATP5B Antibody (E-1) Alexa Fluor® 594 | sc-55597 AF594 | 200 µg/ml | $350.00 | |||
ATP5B Antibody (E-1) Alexa Fluor® 647 | sc-55597 AF647 | 200 µg/ml | $350.00 | |||
ATP5B Antibody (E-1) Alexa Fluor® 680 | sc-55597 AF680 | 200 µg/ml | $350.00 | |||
ATP5B Antibody (E-1) Alexa Fluor® 790 | sc-55597 AF790 | 200 µg/ml | $350.00 |