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Bad Antibody (C-7): sc-8044
- Bad Antibody (C-7) is a mouse monoclonal IgG1 κ Bad antibody, cited in 255 publications, provided at 200 µg/ml
- raised against amino acids 1-168 representing full length Bad of human origin
- Bad Antibody (C-7) is recommended for detection of Bad of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Bad Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Bad (C-7): sc-8044.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Bad Antibody (C-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Bad Antibody (C-7) (see ordering information below).
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Bad Antibody (C-7) is an IgG1 κ mouse monoclonal Bad antibody (also designated BCL2 associated agonist of cell death antibody) that detects the Bad protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Bad Antibody (C-7) is available as both the non-conjugated anti-Bad antibody form, as well as multiple conjugated forms of anti-Bad antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis while other members of the Bcl-2 family, such as Bax and Bak, enhance cell death under various conditions. For instance, Bcl-xL represses cell death, while its shorter form, Bcl-xS, promotes apoptosis. A protein designated Bad exhibits homology to Bcl-2 limited to the BH1 and BH2 domains. Bad functions to dimerize with Bcl-xL and with Bcl-2, but not with Bax, Bcl-xS, Mcl-1, A1 or itself. In mammalian cells, Bad binds with greater affinity to Bcl-xL than to Bcl-2 and reverses the death repressor activity of Bcl-xL but not Bcl-2. Dimerization of Bad with Bcl-xL results in displacement of Bax from Bcl-xL: Bax complexes thereby causing restoration of Bax-mediated apoptosis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- ACTX-8, a cytotoxic L-amino acid oxidase isolated from Agkistrodon acutus snake venom, induces apoptosis in Hela cervical cancer cells. | Zhang, L. and Wei, LJ. 2007. Life Sci. 80: 1189-97. PMID: 17275856
- Docetaxel-induced apoptosis of human melanoma is mediated by activation of c-Jun NH2-terminal kinase and inhibited by the mitogen-activated protein kinase extracellular signal-regulated kinase 1/2 pathway. | Mhaidat, NM., et al. 2007. Clin Cancer Res. 13: 1308-14. PMID: 17317842
- BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death. | Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
- Deregulated Bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines. | Nuηez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
- Identification of immunosuppressant-induced apoptosis in a murine B-cell line and its prevention by bcl-x but not bcl-2. | Gottschalk, AR., et al. 1994. Proc Natl Acad Sci U S A. 91: 7350-4. PMID: 7518929
- Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak. | Kiefer, MC., et al. 1995. Nature. 374: 736-9. PMID: 7715731
- Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death. | Yang, E., et al. 1995. Cell. 80: 285-91. PMID: 7834748
- BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. | Yin, XM., et al. 1994. Nature. 369: 321-3. PMID: 8183370
- Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. | Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bad Antibody (C-7) | sc-8044 | 200 µg/ml | $310.00 | |||
Bad Antibody (C-7): m-IgG Fc BP-HRP Bundle | sc-528212 | 200 µg Ab; 10 µg BP | $348.00 | |||
Bad Antibody (C-7): m-IgGκ BP-HRP Bundle | sc-520535 | 200 µg Ab, 40 µg BP | $348.00 | |||
Bad Antibody (C-7): m-IgG1 BP-HRP Bundle | sc-542820 | 200 µg Ab; 20 µg BP | $348.00 | |||
Bad Antibody (C-7) AC | sc-8044 AC | 500 µg/ml, 25% agarose | $408.00 | |||
Bad Antibody (C-7) HRP | sc-8044 HRP | 200 µg/ml | $310.00 | |||
Bad Antibody (C-7) FITC | sc-8044 FITC | 200 µg/ml | $324.00 | |||
Bad Antibody (C-7) PE | sc-8044 PE | 200 µg/ml | $337.00 | |||
Bad Antibody (C-7) Alexa Fluor® 488 | sc-8044 AF488 | 200 µg/ml | $350.00 | |||
Bad Antibody (C-7) Alexa Fluor® 546 | sc-8044 AF546 | 200 µg/ml | $350.00 | |||
Bad Antibody (C-7) Alexa Fluor® 594 | sc-8044 AF594 | 200 µg/ml | $350.00 | |||
Bad Antibody (C-7) Alexa Fluor® 647 | sc-8044 AF647 | 200 µg/ml | $350.00 | |||
Bad Antibody (C-7) Alexa Fluor® 680 | sc-8044 AF680 | 200 µg/ml | $350.00 | |||
Bad Antibody (C-7) Alexa Fluor® 790 | sc-8044 AF790 | 200 µg/ml | $350.00 |