beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8): sc-21713

Na⁺/K⁺-ATPase β1 Antibody (C464.8) is an IgG_2a κ mouse monoclonal Na+/K+-ATPase beta 1 antibody (also designated ATP1B1 antibody, ATPase Na+/K+ Transporting Subunit Beta 1 antibody, Sodium/Potassium-Transporting ATPase Subunit Beta-1 antibody, Sodium Pump Subunit Beta-1 antibody, ATP1B antibody, Sodium/Potassium-Dependent ATPase Subunit Beta-1 antibody, Beta 1-Subunit Of Na(+)K(+)-ATPase antibody, Na K-ATPase Beta-1 antibody, or Adenosinetriphosphatase antibody) that detects the Na+/K+-ATPase beta 1 protein of mouse, rat, human, rabbit and canine origin by WB, IP and IF. Na⁺/K⁺-ATPase β1 Antibody (C464.8) is available as both the non-conjugated anti-Na+/K+-ATPase beta 1 antibody form, as well as multiple conjugated forms of anti-Na+/K+-ATPase beta 1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as a oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Functional role and immunocytochemical localization of the gamma a and gamma b forms of the Na,K-ATPase gamma subunit.  |  Pu, HX., et al. 2001. J Biol Chem. 276: 20370-8. PMID: 11278761
2. Chemical modification as an approach to elucidation of sodium pump structure-function relations.  |  Pedemonte, CH. and Kaplan, JH. 1990. Am J Physiol. 258: C1-23. PMID: 2154108
3. The sodium pump needs its beta subunit.  |  McDonough, AA., et al. 1990. FASEB J. 4: 1598-605. PMID: 2156741
4. Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport.  |  Ackermann, U. and Geering, K. 1990. FEBS Lett. 269: 105-8. PMID: 2167238
5. A proteolipid associated with Na,K-ATPase is not essential for ATPase activity.  |  Hardwicke, PM. and Freytag, JW. 1981. Biochem Biophys Res Commun. 102: 250-7. PMID: 6272791
6. Functional significance of the beta-subunit for heterodimeric P-type ATPases.  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
7. Na+,K(+)-ATPase.  |  Lingrel, JB. and Kuntzweiler, T. 1994. J Biol Chem. 269: 19659-62. PMID: 8051040
8. Subunit requirements for expression of functional sodium pumps in yeast cells.  |  Scheiner-Bobis, G. and Farley, RA. 1994. Biochim Biophys Acta. 1193: 226-34. PMID: 8054343
9. The alpha-subunit of the Na,K-ATPase has catalytic activity independent of the beta-subunit.  |  Blanco, G., et al. 1994. J Biol Chem. 269: 23420-5. PMID: 8089106
10. Expression, targeting, and assembly of functional Na,K-ATPase polypeptides in baculovirus-infected insect cells.  |  DeTomaso, AW., et al. 1993. J Biol Chem. 268: 1470-8. PMID: 8380413
11. Molecular cloning and immunological characterization of the gamma polypeptide, a small protein associated with the Na,K-ATPase.  |  Mercer, RW., et al. 1993. J Cell Biol. 121: 579-86. PMID: 8387529
12. Structure and function of the Na,K pump: ten years of molecular biology.  |  Pressley, TA. 1996. Miner Electrolyte Metab. 22: 264-71. PMID: 8933497

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

beta 1 Sodium Potassium ATPase/ATP1B1 Antibody (C464.8) Specifications

Alternate Product Name

ATP1B1

Product Clone Name

C464.8

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

Epitope Target

Na+/K+-ATPase β1

Epitope Species

rabbit

Product Reactivity/Detection

Na+/K+-ATPase β1

Species Reactivity/Detection

mouse, rat, human, rabbit, canine

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

25 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin