CD154 Antibody (F-1): sc-374635

CD154 Antibody (F-1) is an IgG_2a κ mouse monoclonal CD154 antibody (also designated CD40LG antibody, CD40L antibody, CD40 Ligand antibody, TNF-Related Activation Protein antibody, TRAP antibody, Tumor Necrosis Factor (Ligand) Superfamily Member 5 antibody, T-B Cell-Activating Molecule antibody, CD40 Antigen Ligand antibody, T-Cell Antigen Gp39 antibody, TNFSF5 antibody, HCD40L antibody, CD40-L antibody, Gp39 antibody, HIGM1 antibody, T-BAM antibody, IMD3 antibody, or IGM antibody) that detects the CD154 protein of mouse, rat and human origin by WB, IP, IF and ELISA. CD154 Antibody (F-1) is available as both the non-conjugated anti-CD154 antibody form, as well as multiple conjugated forms of anti-CD154 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Enhanced NFATc1 nuclear occupancy causes T cell activation independent of CD28 costimulation.  |  Pan, M., et al. 2007. J Immunol. 178: 4315-21. PMID: 17371988
2. Resistance to the induction of mixed chimerism in spontaneously diabetic NOD mice depends on the CD40/CD154 pathway and donor MHC disparity.  |  Luo, B., et al. 2007. Ann N Y Acad Sci. 1103: 94-102. PMID: 17376827
3. A novel RING finger protein interacts with the cytoplasmic domain of CD40.  |  Hu, HM., et al. 1994. J Biol Chem. 269: 30069-72. PMID: 7527023
4. Involvement of CRAF1, a relative of TRAF, in CD40 signaling.  |  Cheng, G., et al. 1995. Science. 267: 1494-8. PMID: 7533327
5. B-cell activation by helper T-cell membranes.  |  Kehry, MR. and Hodgkin, PD. 1994. Crit Rev Immunol. 14: 221-38. PMID: 7538767
6. CD40 and its ligand: central players in B lymphocyte survival, growth, and differentiation.  |  Gordon, J. 1995. Blood Rev. 9: 53-6. PMID: 7540904
7. Expression of the CD40 ligand in T lymphocytes and induction of IgE isotype switching.  |  Fuleihan, R., et al. 1995. Int Arch Allergy Immunol. 107: 43-4. PMID: 7542098
8. TRAF2-mediated activation of NF-kappa B by TNF receptor 2 and CD40.  |  Rothe, M., et al. 1995. Science. 269: 1424-7. PMID: 7544915
9. A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor.  |  Rothe, M., et al. 1994. Cell. 78: 681-92. PMID: 8069916

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD154 Antibody (F-1) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CD154 Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD154 Antibody (F-1) Specifications

Alternate Product Name

CD40L

Product Clone Name

F-1

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

231-267

Epitope Target

CD154

Epitope Species

human

Product Reactivity/Detection

CD154

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

5 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
• m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
• m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
• m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
• m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
• m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
• m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
• m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin