Bax Antibody (B-9): sc-7480

Bax Antibody (B-9) is an IgG_2b κ mouse monoclonal Bax antibody (also designated BCL2 associated X antibody or BCL2L4 antibody) that detects the Bax protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Bax Antibody (B-9) is available as both the non-conjugated anti-Bax antibody form, as well as multiple conjugated forms of anti-Bax antibody, including agarose, PE, FITC and multiple Alexa Fluor^® conjugates. The Bax protein both homodimerizes and forms heterodimers with Bcl-2. Although Bax has extensive amino acid homology with a related protein designated Bcl-2, the Bax Antibody (B-9) specifically detects Bax without showing cross reactivity with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activity of Bcl-2. The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.

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References:

1. Aldose reductase mediates endotoxin-induced production of nitric oxide and cytotoxicity in murine macrophages.  |  Ramana, KV., et al. 2007. Free Radic Biol Med. 42: 1290-302. PMID: 17382209
2. Structure of M11L: A myxoma virus structural homolog of the apoptosis inhibitor, Bcl-2.  |  Douglas, AE., et al. 2007. Protein Sci. 16: 695-703. PMID: 17384234
3. Expression of Bcl-xL, Bax, and p53 in primary tumors and lymph node metastases in oral squamous cell carcinoma.  |  Baltaziak, M., et al. 2006. Ann N Y Acad Sci. 1090: 18-25. PMID: 17384243
4. Alterations in mRNA expression of apoptosis-related genes BCL2, BAX, FAS, caspase-3, and the novel member BCL2L12 after treatment of human leukemic cell line HL60 with the antineoplastic agent etoposide.  |  Floros, KV., et al. 2006. Ann N Y Acad Sci. 1090: 89-97. PMID: 17384250
5. BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death.  |  Hockenbery, DM., et al. 1991. Proc Natl Acad Sci U S A. 88: 6961-5. PMID: 1871110
6. Deregulated Bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines.  |  Nuηez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
7. The bcl-2 candidate proto-oncogene product is a 24-kilodalton integral-membrane protein highly expressed in lymphoid cell lines and lymphomas carrying the t(14;18) translocation.  |  Chen-Levy, Z., et al. 1989. Mol Cell Biol. 9: 701-10. PMID: 2651903
8. Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells.  |  Vaux, DL., et al. 1988. Nature. 335: 440-2. PMID: 3262202
9. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18.  |  Bakhshi, A., et al. 1985. Cell. 41: 899-906. PMID: 3924412
10. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death.  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Bax Antibody (B-9) has 1,423 citations in a variety of scientific publications.

Primary antibodies like Anti-Bax Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Bax Antibody (B-9) Specifications

Alternate Product Name

Bax

Product Clone Name

B-9

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2b kappa light chain

Amino Acids

1-171

Epitope Target

Bax

Epitope Species

mouse

Product Reactivity/Detection

Baxα and Baxβ

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), FCM

Vialing Information

Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

1,423 citations in various scientific publications

Recommended Secondary Detection Reagents


Mouse IgG_2b Binding Proteins

• m-IgG_2b BP-HRP: sc-542741 (Mouse IgG_2b binding protein conjugated to horseradish peroxidase)
• m-IgG_2b BP-B: sc-542744 (Mouse IgG_2b binding protein conjugated to biotin)
• m-IgG_2b BP-FITC: sc-542743 (Mouse IgG_2b binding protein conjugated to fluorescein isothiocyanate)
• m-IgG_2b BP-CFL 488: sc-542745 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 488)
• m-IgG_2b BP-CFL 555: sc-542746 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 555)
• m-IgG_2b BP-CFL 594: sc-542747 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 594)
• m-IgG_2b BP-CFL 647: sc-542748 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 647)
• m-IgG_2b BP-CFL 680: sc-542749 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 680)
• m-IgG_2b BP-CFL 790: sc-542750 (Mouse IgG_2b binding protein conjugated to CruzFluor™ 790)
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Mouse IgG Kappa Light Chain Binding Proteins
• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)



Available Conjugates/Forms




AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin, TRITC: Rhodamine




Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-Bax Antibody (B-9) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), FCM (Flow Cytometry).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.