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Bax Antibody (6D149): sc-70405

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Datasheets
  • Bax Antibody (6D149) is a mouse monoclonal IgG1 κ, cited in 15 publications, provided at 200 µg/ml
  • recommended for detection of Bax of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
  • available conjugated to agarose for IP; and to phycoerythrin for IF, IHC(P) and FCM
  • See Bax (B-9): sc-7480 for Bax antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Bax Antibody (6D149) for WB and IHC(P) applications. These reagents are now offered in bundles with Bax Antibody (6D149) (see ordering information below).

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The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax (Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activity of Bcl-2.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Bax Antibody (6D149)

sc-70405
200 µg/ml
$310.00

Bax Antibody (6D149): m-IgG Fc BP-HRP Bundle

sc-539272
200 µg Ab; 10 µg BP
$348.00

Bax Antibody (6D149): m-IgG1 BP-HRP Bundle

sc-541370
200 µg Ab; 20 µg BP
$348.00

Bax Antibody (6D149) AC

sc-70405 AC
500 µg/ml, 25% agarose
$408.00

Bax Antibody (6D149) PE

sc-70405 PE
200 µg/ml
$337.00

I am using Bax (6D149): sc-70405 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works wellI'm happy to recommend it and think it works well in WB.
Date published: 2016-12-04
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Bax Antibody (6D149) is rated 3.5 out of 5 by 2.
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