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CD3-δ Antibody (F-1): sc-137137
- CD3-δ Antibody (F-1) is a mouse monoclonal IgG2a κ CD3-δ antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 1-171 representing full length CD3-δ of human origin
- CD3-delta Antibody (F-1) is recommended for detection of CD3-δ of human origin by WB, IP, IF and ELISA
- Anti-CD3-delta Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD3-δ (F-1): sc-137137.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD3-δ Antibody (F-1) for WB applications. These reagents are now offered in bundles with CD3-δ Antibody (F-1) (see ordering information below).
CD3-δ Antibody (F-1) is an IgG2a κ mouse monoclonal CD3-delta antibody (also designated CD3D antibody, or CD3 delta antibody) that detects the CD3-delta protein of human origin by WB, IP, IF and ELISA. CD3-δ Antibody (F-1) is available as both the non-conjugated anti-CD3-delta antibody form, as well as multiple conjugated forms of anti-CD3-delta antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (γε), a heterodimer of delta and epsilon chains (δε) and a homodimer of two zeta chains (ζζ) or a heterodimer of zeta and eta chains (ζη). The ζ and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The γ, ε and δ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the ζ chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the ζ chain ITAMs has been solved.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Structure, assembly and intracellular transport of the T cell receptor for antigen. | Exley, M., et al. 1991. Semin Immunol. 3: 283-97. PMID: 1686832
- Signal transduction by the T cell antigen receptor. | Weiss, A., et al. 1991. Semin Immunol. 3: 313-24. PMID: 1839225
- Signal transduction. Zapping tandem SH2 domains. | Weiss, A. 1995. Nature. 377: 17-8. PMID: 7659151
- Molecular basis for interaction of the protein tyrosine kinase ZAP-70 with the T-cell receptor. | Hatada, MH., et al. 1995. Nature. 377: 32-8. PMID: 7659156
- Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins. | Neumeister, EN., et al. 1995. Mol Cell Biol. 15: 3171-8. PMID: 7760813
- Different cytoplasmic structure of the CD3 zeta family dimer modulates the activation signal and function of T cells. | Aoe, T., et al. 1994. Int Immunol. 6: 1671-9. PMID: 7865460
- The role of protein tyrosine kinases and protein tyrosine phosphatases in T cell antigen receptor signal transduction. | Chan, AC., et al. 1994. Annu Rev Immunol. 12: 555-92. PMID: 8011291
- Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand. | Ohno, H., et al. 1994. EMBO J. 13: 1157-65. PMID: 8131747
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD3-δ Antibody (F-1) | sc-137137 | 200 µg/ml | $310.00 | |||
CD3-δ Antibody (F-1): m-IgG Fc BP-HRP Bundle | sc-528867 | 200 µg Ab; 10 µg BP | $348.00 | |||
CD3-δ Antibody (F-1): m-IgGκ BP-HRP Bundle | sc-521435 | 200 µg Ab, 40 µg BP | $348.00 | |||
CD3-δ Antibody (F-1): m-IgG2a BP-HRP Bundle | sc-547195 | 200 µg Ab; 10 µg BP | $348.00 | |||
CD3-δ Antibody (F-1) AC | sc-137137 AC | 500 µg/ml, 25% agarose | $408.00 | |||
CD3-δ Antibody (F-1) HRP | sc-137137 HRP | 200 µg/ml | $310.00 | |||
CD3-δ Antibody (F-1) FITC | sc-137137 FITC | 200 µg/ml | $324.00 | |||
CD3-δ Antibody (F-1) PE | sc-137137 PE | 200 µg/ml | $337.00 | |||
CD3-δ Antibody (F-1) Alexa Fluor® 488 | sc-137137 AF488 | 200 µg/ml | $350.00 | |||
CD3-δ Antibody (F-1) Alexa Fluor® 546 | sc-137137 AF546 | 200 µg/ml | $350.00 | |||
CD3-δ Antibody (F-1) Alexa Fluor® 594 | sc-137137 AF594 | 200 µg/ml | $350.00 | |||
CD3-δ Antibody (F-1) Alexa Fluor® 647 | sc-137137 AF647 | 200 µg/ml | $350.00 | |||
CD3-δ Antibody (F-1) Alexa Fluor® 680 | sc-137137 AF680 | 200 µg/ml | $350.00 | |||
CD3-δ Antibody (F-1) Alexa Fluor® 790 | sc-137137 AF790 | 200 µg/ml | $350.00 |