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CD32-A/B/C Antibody (B-4): sc-166711

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Datasheets
  • CD32-A/B/C Antibody (B-4) is a mouse monoclonal IgG2b κ CD32-A/B/C antibody, cited in 8 publications, provided at 200 µg/ml
  • raised against amino acids 1-206 mapping at the N-terminus of CD32 of human origin
  • CD32-A/B/C Antibody (B-4) is recommended for detection of CD32-A, CD32-B and CD32-C of human origin and Fc γ RIIb and Fc γ RIII of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-CD32-A/B/C Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD32-A/B/C (B-4): sc-166711.
  • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD32-A/B/C Antibody (B-4) for WB and IHC(P) applications. These reagents are now offered in bundles with CD32-A/B/C Antibody (B-4) (see ordering information below).

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CD32-A/B/C Antibody (B-4) is an IgG2b κ mouse monoclonal CD32-A/B/C antibody (also designated FCGR2 antibody, FCGR2A antibody, FCGR2B antibody, FCGR2C antibody, Fc Fragment Of IgG Receptor II antibody, CDw32 antibody, IGFR2 antibody, CD32 antibody, Low Affinity Immunoglobulin Gamma Fc Region Receptor II antibody, Fc Fragment Of IgG Low Affinity 2 Receptor (CD32) antibody, Immunoglobulin G Fc Receptor II antibody, Fc-Gamma-RIIa antibody, FcRII-A antibody, CD32A antibody, FCG2 antibody, or Fc Fragment Of IgG Low Affinity IIa Receptor (CD32) antibody) that detects the CD32-A/B/C protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. CD32-A/B/C Antibody (B-4) is available as both the non-conjugated anti-CD32-A/B/C antibody form, as well as multiple conjugated forms of anti-CD32-A/B/C antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. CD32 (also designated Fc gamma RII) is a low affinity receptor for the Fc fragment of aggregated IgG (1,2). CD32 is responsible for the clearance of immunocomplexes by macrophages and also plays an important role in the regulation of antibody production by B cells (1-4). IgG can noncooperatively bind either one or two highly glycosylated CD32 molecules, and this binding delivers a negative signal for B cells (1,2,5). CD32 exists as several isoforms that are produced by alternative splicing of three distinct genes, A, B, and C (2,6). These isoforms are designated FcgRIIA, FcgRIIB1, FcgRIIB3, and FcgRIIC (1,2,6). All isoforms are present on monocytes, placental trophoblasts and endothelial cells (1,6). In addition, the FcgRIIB forms are present on B lymphocytes, and the FcgRIIA and FcgRIIC forms are found on neutrophils (1,6).

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

CD32-A/B/C Antibody (B-4)

sc-166711
200 µg/ml
$310.00

CD32-A/B/C Antibody (B-4): m-IgGκ BP-HRP Bundle

sc-521718
200 µg Ab, 40 µg BP
$348.00

CD32-A/B/C Antibody (B-4): m-IgG2b BP-HRP Bundle

sc-548958
200 µg Ab; 10 µg BP
$348.00

CD32-A/B/C Antibody (B-4) AC

sc-166711 AC
500 µg/ml, 25% agarose
$408.00

CD32-A/B/C Antibody (B-4) HRP

sc-166711 HRP
200 µg/ml
$310.00

CD32-A/B/C Antibody (B-4) FITC

sc-166711 FITC
200 µg/ml
$324.00

CD32-A/B/C Antibody (B-4) PE

sc-166711 PE
200 µg/ml
$337.00

CD32-A/B/C Antibody (B-4) Alexa Fluor® 488

sc-166711 AF488
200 µg/ml
$350.00

CD32-A/B/C Antibody (B-4) Alexa Fluor® 546

sc-166711 AF546
200 µg/ml
$350.00

CD32-A/B/C Antibody (B-4) Alexa Fluor® 594

sc-166711 AF594
200 µg/ml
$350.00

CD32-A/B/C Antibody (B-4) Alexa Fluor® 647

sc-166711 AF647
200 µg/ml
$350.00

CD32-A/B/C Antibody (B-4) Alexa Fluor® 680

sc-166711 AF680
200 µg/ml
$350.00

CD32-A/B/C Antibody (B-4) Alexa Fluor® 790

sc-166711 AF790
200 µg/ml
$350.00

Is there any issue in using sc-166711: CD32-A/B/C (B-4) mouse monoclonal primary antibody on brain rat samples?

Asked by: MKLM
Thank you for your question. CD32-A/B/C Antibody (B-4): sc-166711 is recommended for detection of CD32-A, CD32-B and CD32-C of human origin and Fc γ RIIb and Fc γ RIII of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA and can also be used on rat brain samples.
Answered by: Tech Support Europe
Date published: 2022-12-14

sc-166711这个产品可以特异性结合大鼠皮层中的M1型小胶质细胞吗

Asked by: banxia
感谢您的提问。如果您能直接拨打(+86) 021-60936350,或者发邮件到我们亚洲服务部的邮箱[email protected]我们就这个问题和其他相关问题进行更多的互动讨论,那将会很有帮助。 Thank you for your question.It would be helpful if you could call us directly at (+86) 021-60936350 or email our Asia Service department at [email protected] for more interactive discussions on this and other related issues.
Answered by: Zhanglong
Date published: 2021-08-31

Are there any issues with using sc-166711: CD32-A/B/C (B-4) mouse monoclonal primary antibody on mouse samples?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-166711 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from WB with none spicific bandWe checked the expression of CD32 in human samples, but there were many none spicific bands.
Date published: 2016-12-14
Rated 5 out of 5 by from Nice Western Blot data of CD32 expression in nonNice Western Blot data of CD32 expression in non-transfected and human CD32 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-08
Rated 5 out of 5 by from Satisfactory Western Blot data of CD32Satisfactory Western Blot data of CD32-A/B/C expression in AML-193 whole cell lysate. -SCBT QC
Date published: 2013-11-30
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CD32-A/B/C Antibody (B-4) is rated 4.3 out of 5 by 3.
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