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Bcl-xS/L Antibody (4H33): sc-70418
- Bcl-xS/L Antibody (4H33) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
- raised against an N-terminal peptide (amino acids 3-14) common to human and mouse Bcl-xL and Bcl-xS
- recommended for detection of Bcl-xL and Bcl-xs of mouse, rat and human origin by WB, IP, IF and IHC(P)
- See Bcl-xL (H-5): sc-8392 for Bcl-xS/L antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Bcl-xS/L Antibody (4H33) for WB and IHC(P) applications. These reagents are now offered in bundles with Bcl-xS/L Antibody (4H33) (see ordering information below).
The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18) bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. A second protein, designated Bcl-associated X protein (Bax) p21, has extensive amino acid homology with Bcl-2 and both homodimerizes and heterodimerizes with Bcl-2. Over-expression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Bcl-x, one of several additional proteins with sequence homology to Bcl-2, is expressed as Bcl-xL, a 233 amino acid protein with 43% sequence identity with Bcl-2 that suppresses cell death, and Bcl-xS, a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188) found in Bcl-xL and which functions as a dominant inhibitor of Bcl-2. A further apoptosis-inducing protein, Bad, dimerizes both with Bcl-xL and to a lesser extent with Bcl-2, thus displacing Bax and inducing apoptosis.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Bcl-xS/L Antibody (4H33) | sc-70418 | 200 µg/ml | $310.00 | |||
Bcl-xS/L Antibody (4H33): m-IgG Fc BP-HRP Bundle | sc-539276 | 200 µg Ab; 10 µg BP | $348.00 | |||
Bcl-xS/L Antibody (4H33): m-IgG2a BP-HRP Bundle | sc-546687 | 200 µg Ab; 10 µg BP | $348.00 |