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CD68 Antibody (3F103): sc-70761

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Datasheets
  • CD68 Antibody (3F103) is a mouse monoclonal IgG1 κ, cited in 48 publications, provided at 200 µg/ml
  • raised against a subcellular fraction of human alveolar macrophages
  • recommended for detection of CD68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD68 (3F103): sc-70761.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD68 Antibody (3F103) for WB and IHC(P) applications. These reagents are now offered in bundles with CD68 Antibody (3F103) (see ordering information below).

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CD68 Antibody (3F103) is an IgG1 κ mouse monoclonal CD68 antibody that detects CD68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. CD68 Antibody (3F103) is available as the non-conjugated anti-CD68 antibody form. CD68, which is homologous to the mouse antigen macrosialin, belongs to a family of acidic, highly glycosylated lysosomal glycoproteins (LGPs) that includes lamp-1 and lamp-2. CD68 is found in cytoplasmic granules and in the cytoplasm of various non-hematopoietic tissues including liver and kidney tubules and glomeruli. CD68 is also found, to a lesser extent, on the surface of macrophages, monocytes, neutrophils, basophils and large lymphocytes. LGPs are major components of lysosomal membranes and may act to protect the membranes from attack by hydrolases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

CD68 Antibody (3F103)

sc-70761
200 µg/ml
$310.00

CD68 Antibody (3F103): m-IgG Fc BP-HRP Bundle

sc-528652
200 µg Ab; 10 µg BP
$348.00

CD68 Antibody (3F103): m-IgGκ BP-HRP Bundle

sc-521091
200 µg Ab, 40 µg BP
$348.00

CD68 Antibody (3F103): m-IgG1 BP-HRP Bundle

sc-543054
200 µg Ab; 20 µg BP
$348.00

What concentration for Flow cytometry is proper to use? It is suggested in the datasheet to use 1 ug/million cells, however, what concentration of Ab is recommended?

Asked by: Anonymous
Thank you for your question. This antibody, CD68 antibody, sc-70761, is provided as 200µg in 1ml, so if you need to use 1µg you will need to take 5µl of antibody solution. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-11-19

Which is the volume of the vial? Because it is referenced as 200 µg/ml but recommended concentrations are not in µg/ml

Asked by: VíctorSrX
Thank you for your question. CD68 Antibody (3F103) is a mouse monoclonal IgG1 provided at 200 µg in 1 mL volume.
Answered by: Technical Support
Date published: 2017-02-09

What is the best secondary to use with this antibody in Western Blot?

Asked by: Anna99
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-17

Anyone knows which is the appropriate pH for Antigen Retrieval?

Asked by: VíctorSrX
Thank you for your question. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-11
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Rated 1 out of 5 by from Not good for WB; too faint even at 1:200THP1 macrophages and Raw macrophages showed very faint bands to none when loaded15- 80ug proteins/well for WB; used 1:200 at Room temp after repeated use of this antibody for 2 days in cold.
Date published: 2018-12-20
Rated 4 out of 5 by from Good ABThis antibody gave a good result in IHC on frozen human section in a dilution of 1:100.
Date published: 2018-04-03
Rated 5 out of 5 by from great for TAM detectingI am very glad to receive your prdoucts two weeks ago.we use this antibody to mark the monocytes cells isolated from the peripheral blood very well, which is very helpful to our research.
Date published: 2017-12-04
Rated 5 out of 5 by from CD 68 immunohistochemical staining for TAMsIt worked for our tumor tissue biopsy sample. I got excellent result in immunohistochemical staining by using this CD68 antibody for our tumor-associated macrophage study.
Date published: 2017-12-04
Rated 3 out of 5 by from for WB analysisThis antibody tried many times,at first no band.I tried different dilutions, different expose time,finally tried 1:200 and incubated 2hour at RT, however the band still not strong and the antibody conceration is a little low.
Date published: 2017-06-25
Rated 5 out of 5 by from Great CD68 antibodyCD68 (3F103) works great for WB and IF. Specific IF staining of methanol-fixed HeLa cells showing membrane localization.
Date published: 2017-01-11
Rated 5 out of 5 by from CD 68 immunohistochemical staining for SCLCIt worked for our FFPE format lymph node and lung tissue biopsy sample. We got excellent result in immunohistochemical staining by using this CD68 antibody for our macrophage phenotypic study.
Date published: 2016-12-13
Rated 5 out of 5 by from Highly recommendable antibody in IHC PHighly recommendable antibody in IHC P
Date published: 2015-12-07
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CD68 Antibody (3F103) is rated 4.5 out of 5 by 13.
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