CD88 Antibody (B-6): sc-271949

CD88 Antibody (B-6) is an IgG1 κ mouse monoclonal CD88 antibody that detects CD88 of human origin by WB, IP, IF, IHC(P) and ELISA. CD88 Antibody (B-6) is available as the non-conjugated anti-CD88 antibody form, as well as multiple conjugated forms of anti-CD88 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. CD88, also known as C5a receptor (C5aR), is a G protein-coupled integral membrane protein. CD88, which is expressed on neutrophils, monocytes, macrophages, hepatocytes and mast cells, as well as on various epithelial and endothelial cells, serves as a receptor for the inflammatory peptide C5a. Research studies suggest a role for CD88 in the inflammatory response. The binding of C5a to CD88 has been shown to elicit increased production of acute phase proteins in liver. In brain, an increased production of CD88 has been shown to be associated with inflammation. Research also indicates a role for C5a/C5aR in the pathogenesis of rheumatoid arthritis, as well as a heightened responsiveness of human bronchial epithelial cells (HBECs) to C5a upon exposure of these cells to cigarette smoke and other environmental irritants.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Human T cells express the C5a receptor and are chemoattracted to C5a.  |  Nataf, S., et al. 1999. J Immunol. 162: 4018-23. PMID: 10201923
2. The chemotactic receptor for human C5a anaphylatoxin.  |  Gerard, NP. and Gerard, C. 1991. Nature. 349: 614-7. PMID: 1847994
3. Anaphylatoxins: C3a and C5a.  |  Hugli, TE. and Mυller-Eberhard, HJ. 1978. Adv Immunol. 26: 1-53. PMID: 358800
4. Differential expression of complement receptors on human basophils and mast cells. Evidence for mast cell heterogeneity and CD88/C5aR expression on skin mast cells.  |  Fυreder, W., et al. 1995. J Immunol. 155: 3152-60. PMID: 7673728
5. Cellular expression of the C5a anaphylatoxin receptor (C5aR): demonstration of C5aR on nonmyeloid cells of the liver and lung.  |  Haviland, DL., et al. 1995. J Immunol. 154: 1861-9. PMID: 7836770
6. Detection of C5a receptors on human eosinophils and inhibition of eosinophil effector functions by anti-C5a receptor (CD88) antibodies.  |  Elsner, J., et al. 1996. Eur J Immunol. 26: 1560-4. PMID: 8766561
7. Expression of the receptor for complement C5a (CD88) is up-regulated on reactive astrocytes, microglia, and endothelial cells in the inflamed human central nervous system.  |  Gasque, P., et al. 1997. Am J Pathol. 150: 31-41. PMID: 9006319
8. Expression of the C5a receptor (CD88) on synovial mast cells in patients with rheumatoid arthritis.  |  Kiener, HP., et al. 1998. Arthritis Rheum. 41: 233-45. PMID: 9485081
9. Expression of receptors for C5a anaphylatoxin (CD88) on human bronchial epithelial cells: enhancement of C5a-mediated release of IL-8 upon exposure to cigarette smoke.  |  Floreani, AA., et al. 1998. J Immunol. 160: 5073-81. PMID: 9590258

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD88 Antibody (B-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CD88 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD88 Antibody (B-6) Specifications

Alternate Product Name

CD88

Product Clone Name

B-6

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

251-350

Epitope Target

CD88

Epitope Species

human

Product Reactivity/Detection

CD88

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

2 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
• m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
• m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
• m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
• m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
• m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
• m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
• m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin