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C3 Antibody (B-9): sc-28294
- C3 Antibody (B-9) is a mouse monoclonal IgG1 κ C3 antibody, cited in 56 publications, provided at 200 µg/ml
- raised against amino acids 541-840 of C3 of human origin
- C3 Antibody (B-9) is recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-C3 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of C3 (B-9): sc-28294.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for C3 Antibody (B-9) for WB and IHC(P) applications. These reagents are now offered in bundles with C3 Antibody (B-9) (see ordering information below).
C3 Antibody (B-9) is an IgG1 κ mouse monoclonal C3 antibody (also designated C3 antibody or Complement C3 antibody) that detects C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. C3 Antibody (B-9) is available as the non-conjugated anti-C3 antibody form, as well as multiple conjugated forms of anti-C3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Complement C3 precursor contains complement C3 beta chain, complement C3 alpha chain, C3a anaphylatoxin, complement C3b alpha chain, complement C3c fragment, complement C3dg fragment, complement C3g fragment, complement C3d fragment and complement C3f fragment. C3a, C4a, and C5a are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage that aids in the elimination of pathogens. C3a and C5a secretion correlates with pathophysiological phenotypes such as asthma and bacterial meningitis. Binding of these proteins to their respective G protein-coupled receptors (C3aR, C5aR), which are present on the surface of myeloid leukocytes, induces proinflammatory events such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation and cellular chemotaxis. C3aR is expressed in brain and activated B-lymphocytes whereas C5aR is prevalent on the surface of hepatocyte, lung, smooth muscle, and endothelial cells. Upon activation, C3aR and C5aR are susceptible to rapid GRK-mediated phosphorylation and clathrin-coated vesicle targeting. C5aR utilizes the Ras-Raf-ERK1/2 cascade and couples to Gi/G16 proteins.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Modulation of C3a activity: internalization of the human C3a receptor and its inhibition by C5a. | Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
- Ligand-induced phosphorylation of anaphylatoxin receptors C3aR and C5aR is mediated by ′G protein-coupled receptor kinases. | Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
- A role for the C3a anaphylatoxin receptor in the effector phase of asthma. | Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
- Human complement component C3: cDNA coding sequence and derived primary structure. | de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
- Mitogen-activated protein kinase activation requires two signal inputs from the human anaphylatoxin C5a receptor. | Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
- TNF-alpha-mediated expression of the receptor for anaphylatoxin C5a on neurons in experimental Listeria meningoencephalitis. | Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C3 Antibody (B-9) | sc-28294 | 200 µg/ml | $310.00 | |||
C3 Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-528410 | 200 µg Ab; 10 µg BP | $348.00 | |||
C3 Antibody (B-9): m-IgGκ BP-HRP Bundle | sc-520784 | 200 µg Ab, 40 µg BP | $348.00 | |||
C3 Antibody (B-9) AC | sc-28294 AC | 500 µg/ml, 25% agarose | $408.00 | |||
C3 Antibody (B-9) HRP | sc-28294 HRP | 200 µg/ml | $310.00 | |||
C3 Antibody (B-9) FITC | sc-28294 FITC | 200 µg/ml | $324.00 | |||
C3 Antibody (B-9) PE | sc-28294 PE | 200 µg/ml | $337.00 | |||
C3 Antibody (B-9) Alexa Fluor® 488 | sc-28294 AF488 | 200 µg/ml | $350.00 | |||
C3 Antibody (B-9) Alexa Fluor® 546 | sc-28294 AF546 | 200 µg/ml | $350.00 | |||
C3 Antibody (B-9) Alexa Fluor® 594 | sc-28294 AF594 | 200 µg/ml | $350.00 | |||
C3 Antibody (B-9) Alexa Fluor® 647 | sc-28294 AF647 | 200 µg/ml | $350.00 | |||
C3 Antibody (B-9) Alexa Fluor® 680 | sc-28294 AF680 | 200 µg/ml | $350.00 | |||
C3 Antibody (B-9) Alexa Fluor® 790 | sc-28294 AF790 | 200 µg/ml | $350.00 |