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CTLA-4 Antibody (F-8): sc-376016
- CTLA-4 Antibody (F-8) is a mouse monoclonal IgG1 κ CTLA-4 antibody, cited in 56 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 195-223 at the C-terminus of CTLA-4 of human origin
- CTLA-4 Antibody (F-8) is recommended for detection of CTLA-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-CTLA-4 Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CTLA-4 (F-8): sc-376016.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CTLA-4 Antibody (F-8) for WB and IHC(P) applications. These reagents are now offered in bundles with CTLA-4 Antibody (F-8) (see ordering information below).
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CTLA-4 Antibody (F-8) is an IgG1 κ mouse monoclonal CTLA-4 antibody (also designated CD152 antibody or CTLA4 antibody) that detects the CTLA-4 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. CTLA-4 Antibody (F-8) is available as both the non-conjugated anti-CTLA-4 antibody form, as well as multiple conjugated forms of anti-CTLA-4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells, bind the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Costimulation of T lymphocytes: the role of CD28, CTLA-4, and B7/BB1 in interleukin-2 production and immunotherapy. | Schwartz, RH. 1992. Cell. 71: 1065-8. PMID: 1335362
- Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7. | Freeman, GJ., et al. 1991. J Exp Med. 174: 625-31. PMID: 1714935
- Regulation of CD28 expression on CD8+ T cells by CTLA-4. | Berg, M. and Zavazava, N. 2008. J Leukoc Biol. 83: 853-63. PMID: 18162511
- Potential roles of the B7 and CD28 receptor families in autoimmunity and immune evasion. | Harlan, DM., et al. 1995. Clin Immunol Immunopathol. 75: 99-111. PMID: 7535672
- Identification of residues in the V domain of CD80 (B7-1) implicated in functional interactions with CD28 and CTLA4. | Fargeas, CA., et al. 1995. J Exp Med. 182: 667-75. PMID: 7544393
- Both extracellular immunoglobin-like domains of CD80 contain residues critical for binding T cell surface receptors CTLA-4 and CD28. | Peach, RJ., et al. 1995. J Biol Chem. 270: 21181-7. PMID: 7545666
- A novel receptor involved in T-cell activation. | Cocks, BG., et al. 1995. Nature. 376: 260-3. PMID: 7617038
- CTLA4 mediates antigen-specific apoptosis of human T cells. | Gribben, JG., et al. 1995. Proc Natl Acad Sci U S A. 92: 811-5. PMID: 7846057
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTLA-4 Antibody (F-8) | sc-376016 | 200 µg/ml | $310.00 | |||
CTLA-4 Antibody (F-8): m-IgG Fc BP-HRP Bundle | sc-529801 | 200 µg Ab; 10 µg BP | $348.00 | |||
CTLA-4 Antibody (F-8): m-IgGκ BP-HRP Bundle | sc-522818 | 200 µg Ab, 40 µg BP | $348.00 | |||
CTLA-4 Antibody (F-8): m-IgG1 BP-HRP Bundle | sc-543694 | 200 µg Ab; 20 µg BP | $348.00 | |||
CTLA-4 Antibody (F-8) AC | sc-376016 AC | 500 µg/ml, 25% agarose | $408.00 | |||
CTLA-4 Antibody (F-8) HRP | sc-376016 HRP | 200 µg/ml | $310.00 | |||
CTLA-4 Antibody (F-8) FITC | sc-376016 FITC | 200 µg/ml | $324.00 | |||
CTLA-4 Antibody (F-8) PE | sc-376016 PE | 200 µg/ml | $337.00 | |||
CTLA-4 Antibody (F-8) Alexa Fluor® 488 | sc-376016 AF488 | 200 µg/ml | $350.00 | |||
CTLA-4 Antibody (F-8) Alexa Fluor® 546 | sc-376016 AF546 | 200 µg/ml | $350.00 | |||
CTLA-4 Antibody (F-8) Alexa Fluor® 594 | sc-376016 AF594 | 200 µg/ml | $350.00 | |||
CTLA-4 Antibody (F-8) Alexa Fluor® 647 | sc-376016 AF647 | 200 µg/ml | $350.00 | |||
CTLA-4 Antibody (F-8) Alexa Fluor® 680 | sc-376016 AF680 | 200 µg/ml | $350.00 | |||
CTLA-4 Antibody (F-8) Alexa Fluor® 790 | sc-376016 AF790 | 200 µg/ml | $350.00 | |||
CTLA-4 (F-8) Neutralizing Peptide | sc-376016 P | 100 µg/0.5 ml | $67.00 |