Glycophorin C/D Antibody (F-8): sc-365599

Glycophorin C/D Antibody (F-8) is an IgG_2b κ mouse monoclonal Glycophorin C/D antibody (also designated Glycophorin C/D antibody) that detects the Glycophorin C/D protein of human origin by WB, IP, IF and ELISA. Glycophorin C/D Antibody (F-8) is available as both the non-conjugated anti-Glycophorin C/D antibody form, as well as multiple conjugated forms of anti-Glycophorin C/D antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Glycophorins A, B and C are sialoglycoproteins of the human erythrocyte membrane, which bear the antigenic determinants for the MN, Ss and Gerbich blood groups, respectively. Glycophorins span the membrane once and present their amino-terminal end to the extracellular surface of the human erythrocyte. The genetic array of expressed glycophorin surface antigens on erythrocytes defines the blood group phenotype of the individual. The human Glycophorin A gene maps to chromosome 4q31.21, contains 7 exons which are 97% homologous to Glycophorin B and encodes a 150 amino acid protein. The human Glycophorin B gene also maps to chromosome 4q31.21 and encodes a 91 amino acid protein. The human Glycophorin C gene maps to chromosome 2q14.3 and contains four exons. Glycophorin C transcript can generate two protein isoforms. Isoform 1 includes all 4 exons and encodes the full length 128 amino acid Glycophorin C protein. Isoform 2, also known as Glycophorin D, is missing exon 2 and encodes a 109 amino acid protein, which specifies the Yus subtype of the Gerbich phenotype.

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References:

1. Regulation of the glycophorin C-protein 4.1 membrane-to-skeleton bridge and evaluation of its contribution to erythrocyte membrane stability.  |  Chang, SH. and Low, PS. 2001. J Biol Chem. 276: 22223-30. PMID: 11294862
2. In vivo detection of hetero-association of glycophorin-A and its mutants within the membrane.  |  Gerber, D. and Shai, Y. 2001. J Biol Chem. 276: 31229-32. PMID: 11402026
3. Glycophorin C is the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl).  |  Lobo, CA., et al. 2003. Blood. 101: 4628-31. PMID: 12576308
4. Distinct regions of human glycophorin A enhance human red cell anion exchanger (band 3; AE1) transport function and surface trafficking.  |  Young, MT. and Tanner, MJ. 2003. J Biol Chem. 278: 32954-61. PMID: 12813056
5. Oligomerization, biogenesis, and signaling is promoted by a glycophorin A-like dimerization motif in transmembrane domain 1 of a yeast G protein-coupled receptor.  |  Overton, MC., et al. 2003. J Biol Chem. 278: 49369-77. PMID: 14506226
6. Altered structure and anion transport properties of band 3 (AE1, SLC4A1) in human red cells lacking glycophorin A.  |  Bruce, LJ., et al. 2004. J Biol Chem. 279: 2414-20. PMID: 14604989
7. SCL assembles a multifactorial complex that determines glycophorin A expression.  |  Lahlil, R., et al. 2004. Mol Cell Biol. 24: 1439-52. PMID: 14749362
8. Structural adaptation of the glycophorin A transmembrane homodimer to D-amino acid modifications.  |  Gerber, D., et al. 2004. J Mol Biol. 339: 243-50. PMID: 15123435
9. Complex interactions at the helix-helix interface stabilize the glycophorin A transmembrane dimer.  |  Doura, AK. and Fleming, KG. 2004. J Mol Biol. 343: 1487-97. PMID: 15491626
10. Influence of hydrophobic matching on association of model transmembrane fragments containing a minimised glycophorin A dimerisation motif.  |  Orzáez, M., et al. 2005. FEBS Lett. 579: 1633-8. PMID: 15757653

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Glycophorin C/D Antibody (F-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Glycophorin C/D Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Glycophorin C/D Antibody (F-8) Specifications

Alternate Product Name

Glycophorin C/D

Product Clone Name

F-8

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2b kappa light chain

Amino Acids

1-85

Epitope Target

Glycophorin C

Epitope Species

human

Product Reactivity/Detection

Glycophorin C and D

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

0 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Kappa Light Chain Binding Proteins

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Glycophorin C antibody is Glycophorin C Antibody (E5): sc-59185.