HLA-D/DR/DQ Antibody (35): sc-53305

HLA-D/DR/DQ Antibody (35) is an IgG₃ κ mouse monoclonal HLA-D/DR/DQ antibody (also designated HLA class II HLAD / HLADR / HLADQ antibody, major histocompatibility complex (MHC) class II (33-35 kDa α)/(26-28 kDa β) heterodimer cell surface receptor antibody, chromosome 6p21 gene complex class 2 D/DR/DQ antibody, or Human Leukocyte Antigen – D/DR/DQ isotypes antibody) that detects the HLA-D/DR/DQ protein of human origin by IP, IF and FCM. HLA-D/DR/DQ Antibody (35) is available as both the non-conjugated anti-HLA-D/DR/DQ antibody form, as well as multiple conjugated forms of anti-HLA-D/DR/DQ antibody, including PE, FITC. Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T-cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in mhc ii-a/b dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, DQ, DP and DR) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. The alpha 1 chain of HLA-DQ1 class II molecule (Ia antigen) complex can bind peptides and present them to CD4+ T lymphocytes.

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References:

1. HLA-DMA and HLA-DMB alleles in German patients with type 1 diabetes mellitus.  |  Siegmund, T., et al. 1999. Tissue Antigens. 54: 291-4. PMID: 10519369
2. The association of HLA-DM genes with rheumatoid arthritis in Eastern France.  |  Toussirot, E., et al. 2000. Hum Immunol. 61: 303-8. PMID: 10689120
3. Functional HLA-DM on the surface of B cells and immature dendritic cells.  |  Arndt, SO., et al. 2000. EMBO J. 19: 1241-51. PMID: 10716924
4. Functional characterization of a lysosomal sorting motif in the cytoplasmic tail of HLA-DObeta.  |  Brunet, A., et al. 2000. J Biol Chem. 275: 37062-71. PMID: 10964920
5. The down-regulation of HLA-DM gene expression in rheumatoid arthritis is not related to their promoter polymorphism.  |  Louis-Plence, P., et al. 2000. J Immunol. 165: 4861-9. PMID: 11046010
6. Determination of the HLA-DM interaction site on HLA-DR molecules.  |  Doebele, RC., et al. 2000. Immunity. 13: 517-27. PMID: 11070170
7. Association of colorectal tumor epithelium expressing HLA-D/DR with CD8-positive T-cells and mononuclear phagocytes.  |  Allen, CA. and Hogg, N. 1987. Cancer Res. 47: 2919-23. PMID: 3105871
8. A role for HLA-DO as a co-chaperone of HLA-DM in peptide loading of MHC class II molecules.  |  Kropshofer, H., et al. 1998. EMBO J. 17: 2971-81. PMID: 9606180

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HLA-D/DR/DQ Antibody (35) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HLA-D/DR/DQ Antibody (35) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HLA-D/DR/DQ Antibody (35) Specifications

Alternate Product Name

human leukocyte antigen (HLA) HLA-D/HLA-DR/HLA-DQ

Product Clone Name

35

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₃ kappa light chain

Amino Acids

Epitope Target

HLA-D/DR/DQ

Epitope Species

mouse

Product Reactivity/Detection

HLA-D, HLA-DR and HLA-DQ

Species Reactivity/Detection

human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

IP, IF, FCM

Vialing Information

Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

1 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

FITC: Fluoroscein, PE: Phycoerythrin

The preferred HLA-DR antibody is HLA-DR Antibody (L243): sc-18875.