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HLA-DRα Antibody (G-7): sc-55593
- HLA-DRα Antibody (G-7) is a mouse monoclonal IgG1 κ HLA-DRα antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 1-254 representing full length HLA-DRa of human origin
- HLA-DR alpha Antibody (G-7) is recommended for detection of HLA-DRa of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-HLA-DR alpha Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of HLA-DRα (G-7): sc-55593.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HLA-DRα Antibody (G-7) for WB and IHC(P) applications. These reagents are now offered in bundles with HLA-DRα Antibody (G-7) (see ordering information below).
HLA-DRα Antibody (G-7) is an IgG1 κ mouse monoclonal HLA-DR alpha antibody (also designated HLA class II alpha chain antibody, major histocompatibility complex (MHC) class II 33-35 kDa α chain antibody, HLA class II alpha (DRA) chain associated antibody, chromosome 6p21 gene complex class 2 DR antibody, Human Leukocyte Antigen – DR isotype antibody, DRA, or DRA1 associated antibody) that detects the HLA-DR alpha protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. HLA-DRα Antibody (G-7) is available as both the non-conjugated anti-HLA-DR alpha antibody form, as well as multiple conjugated forms of anti-HLA-DR alpha antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in mhc ii-a/b dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (β-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- HLA-DMA and HLA-DMB alleles in German patients with type 1 diabetes mellitus. | Siegmund, T., et al. 1999. Tissue Antigens. 54: 291-4. PMID: 10519369
- The association of HLA-DM genes with rheumatoid arthritis in Eastern France. | Toussirot, E., et al. 2000. Hum Immunol. 61: 303-8. PMID: 10689120
- Functional HLA-DM on the surface of B cells and immature dendritic cells. | Arndt, SO., et al. 2000. EMBO J. 19: 1241-51. PMID: 10716924
- Functional characterization of a lysosomal sorting motif in the cytoplasmic tail of HLA-DObeta. | Brunet, A., et al. 2000. J Biol Chem. 275: 37062-71. PMID: 10964920
- The down-regulation of HLA-DM gene expression in rheumatoid arthritis is not related to their promoter polymorphism. | Louis-Plence, P., et al. 2000. J Immunol. 165: 4861-9. PMID: 11046010
- Determination of the HLA-DM interaction site on HLA-DR molecules. | Doebele, RC., et al. 2000. Immunity. 13: 517-27. PMID: 11070170
- Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. | Horejsí, V., et al. 1986. Folia Biol (Praha). 32: 12-25. PMID: 2422063
- Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. | Horejsí, V., et al. 1986. Tissue Antigens. 28: 288-97. PMID: 2435017
- A role for HLA-DO as a co-chaperone of HLA-DM in peptide loading of MHC class II molecules. | Kropshofer, H., et al. 1998. EMBO J. 17: 2971-81. PMID: 9606180
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DRα Antibody (G-7) | sc-55593 | 200 µg/ml | $310.00 | |||
HLA-DRα Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-528606 | 200 µg Ab; 10 µg BP | $348.00 | |||
HLA-DRα Antibody (G-7): m-IgGκ BP-HRP Bundle | sc-521026 | 200 µg Ab, 40 µg BP | $348.00 | |||
HLA-DRα Antibody (G-7): m-IgG1 BP-HRP Bundle | sc-543025 | 200 µg Ab; 20 µg BP | $348.00 | |||
HLA-DRα Antibody (G-7) AC | sc-55593 AC | 500 µg/ml, 25% agarose | $408.00 | |||
HLA-DRα Antibody (G-7) HRP | sc-55593 HRP | 200 µg/ml | $310.00 | |||
HLA-DRα Antibody (G-7) FITC | sc-55593 FITC | 200 µg/ml | $324.00 | |||
HLA-DRα Antibody (G-7) PE | sc-55593 PE | 200 µg/ml | $337.00 | |||
HLA-DRα Antibody (G-7) Alexa Fluor® 488 | sc-55593 AF488 | 200 µg/ml | $350.00 | |||
HLA-DRα Antibody (G-7) Alexa Fluor® 546 | sc-55593 AF546 | 200 µg/ml | $350.00 | |||
HLA-DRα Antibody (G-7) Alexa Fluor® 594 | sc-55593 AF594 | 200 µg/ml | $350.00 | |||
HLA-DRα Antibody (G-7) Alexa Fluor® 647 | sc-55593 AF647 | 200 µg/ml | $350.00 | |||
HLA-DRα Antibody (G-7) Alexa Fluor® 680 | sc-55593 AF680 | 200 µg/ml | $350.00 | |||
HLA-DRα Antibody (G-7) Alexa Fluor® 790 | sc-55593 AF790 | 200 µg/ml | $350.00 |