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PBEF Antibody (F-8): sc-393510

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Datasheets
  • PBEF Antibody (F-8) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 1-300 mapping at the N-terminus of PBEF of human origin
  • recommended for detection of PBEF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PBEF Antibody (F-8) for WB and IHC(P) applications. These reagents are now offered in bundles with PBEF Antibody (F-8) (see ordering information below).

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SEE ALSO...

Pre-B cell-enhancing factor (PBEF), also designated nicotinamide phosphoribosyltransferase (Nampt) or visfatin, belongs to the NAPRTase family of proteins. PBEF may be involved in enhancing the effect of IL-7 and SCF on the formation of early B-lineage precursor colonies. It is involved in the catalysis of nicotinamide with 5-phosphoribosyl-1-pyrophosphate, yielding nicotinamide mononucleotide, which is important in NAD biosynthesis. This is a rate limiting step in the NAD biosynthesis pathway. Highly enriched in the visceral fat of both human and mouse, PBEF expression levels in plasma increase during the development of obesity. PBEF is a cytoplasmic protein expressed primarily in bone marrow, muscle and liver tissue, but it can also be detected in placenta, lung, kidney and heart tissue.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PBEF Antibody (F-8)

sc-393510
200 µg/ml
$310.00

PBEF Antibody (F-8): m-IgG Fc BP-HRP Bundle

sc-538234
200 µg Ab; 10 µg BP
$348.00

PBEF Antibody (F-8): m-IgGκ BP-HRP Bundle

sc-535806
200 µg Ab; 40 µg BP
$348.00

PBEF Antibody (F-8): m-IgG1 BP-HRP Bundle

sc-545593
200 µg Ab; 20 µg BP
$348.00

I am using PBEF (F-8): sc-393510 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Satisfactory Western Blot data of PBEF expressionSatisfactory Western Blot data of PBEF expression in HL-60 whole cell lysate. -SCBT QC
Date published: 2013-10-11
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PBEF Antibody (F-8) is rated 5.0 out of 5 by 2.
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