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N-cadherin Antibody (H-2): sc-393933

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Datasheets
  • N-cadherin Antibody (H-2) is a mouse monoclonal IgG1 κ, cited in 36 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 800-825 within a C-terminal cytoplasmic domain of N-cadherin of human origin
  • Anti-N-cadherin Antibody (H-2) is recommended for detection of N-cadherin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-N-cadherin Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of N-cadherin (H-2): sc-393933.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for N-cadherin Antibody (H-2) for WB and IHC(P) applications. These reagents are now offered in bundles with N-cadherin Antibody (H-2) (see ordering information below).

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SEE ALSO...

N-cadherin Antibody (H-2) is an IgG1 κ mouse monoclonal N-cadherin antibody (also designated N-cadherin antibody or CDH2 antibody) that detects N-cadherin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. N-cadherin Antibody (H-2) is available as the non-conjugated anti-N-cadherin antibody form, as well as multiple conjugated forms of anti-N-cadherin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy-terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

N-cadherin Antibody (H-2)

sc-393933
200 µg/ml
$310.00

N-cadherin Antibody (H-2): m-IgG Fc BP-HRP Bundle

sc-530638
200 µg Ab; 10 µg BP
$348.00

N-cadherin Antibody (H-2): m-IgGκ BP-HRP Bundle

sc-524153
200 µg Ab, 40 µg BP
$348.00

N-cadherin Antibody (H-2): m-IgG1 BP-HRP Bundle

sc-544173
200 µg Ab; 20 µg BP
$348.00

N-cadherin Antibody (H-2) AC

sc-393933 AC
500 µg/ml, 25% agarose
$408.00

N-cadherin Antibody (H-2) HRP

sc-393933 HRP
200 µg/ml
$310.00

N-cadherin Antibody (H-2) FITC

sc-393933 FITC
200 µg/ml
$324.00

N-cadherin Antibody (H-2) PE

sc-393933 PE
200 µg/ml
$337.00

N-cadherin Antibody (H-2) Alexa Fluor® 488

sc-393933 AF488
200 µg/ml
$350.00

N-cadherin Antibody (H-2) Alexa Fluor® 546

sc-393933 AF546
200 µg/ml
$350.00

N-cadherin Antibody (H-2) Alexa Fluor® 594

sc-393933 AF594
200 µg/ml
$350.00

N-cadherin Antibody (H-2) Alexa Fluor® 647

sc-393933 AF647
200 µg/ml
$350.00

N-cadherin Antibody (H-2) Alexa Fluor® 680

sc-393933 AF680
200 µg/ml
$350.00

N-cadherin Antibody (H-2) Alexa Fluor® 790

sc-393933 AF790
200 µg/ml
$350.00

N-cadherin (H-2) Neutralizing Peptide

sc-393933 P
100 µg/0.5 ml
$67.00

Can you recommend a positive controls (besides cardiac muscle) for IHC (P) for this antibody please?

Asked by: Lucytch
Thank you for your question. N-Cadherin is highly expressed in several tissues including brain, muscle, endocrine tissues, liver, and kidney.
Answered by: Tech Support Europe
Date published: 2020-11-19

想问一下怎么申请试用装 sc-393933 AF488 和 N-cadherin Antibody (H-2)

Asked by: pzfei
感谢您的提问。关于试用装的申请,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. For sample request, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-04-23

Can you recommend a positive control to use with N-cadherin (H-2): sc-393933 monoclonal antibody for Western blot?

Asked by: jenni
We recommend mouse brain (sc-2253) and human heart (sc-363763) tissue extracts to use as positive controls as we have great Western blot data showing specific detection of Calnexin in these cell lines.
Answered by: Technical Support 2
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with N-cadherin (H-2): sc-393933?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-12
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Rated 5 out of 5 by from Good for WBGreat for WB at 1:200 dilution. I will also try in IF.
Date published: 2021-06-12
Rated 5 out of 5 by from Great for both IF and WBI tested out the sample version of this antibody for both WB (1:500) and IF (1:250) and got a strong signal. The cell junction localization looks great by both 10x and 20x imaging.
Date published: 2019-02-08
Rated 5 out of 5 by from Good signal (inmunofluorescence)We have used for IF and we obtained a good fluorescence signal (1:50 dilution)
Date published: 2018-03-07
Rated 5 out of 5 by from Great featuresIt's great for my WB. N-cad had changed with my treatment for C4-2 cell line.
Date published: 2017-11-12
Rated 5 out of 5 by from Good jobWe got just a sample to test and it worked so great that now we bought it! It works in WB and IF.
Date published: 2017-11-07
Rated 4 out of 5 by from Nice IFI tested on immunofluorescence and it worked well. nice membrane staining.
Date published: 2017-11-01
Rated 5 out of 5 by from Works wellIt works well and worth recommending~~~~~~~~~~~~~~~~~~~~~~~~~~
Date published: 2017-06-21
Rated 5 out of 5 by from Superb western and IHCStrong clear western blot results; also a great product for IHC staining
Date published: 2017-01-30
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N-cadherin Antibody (H-2) is rated 4.9 out of 5 by 10.
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