Hi, Can PFA 4% be used for fixation?
In particular, regarding Pro-COL1A1 Antibody (N-17): sc-8782
Thanks
Yoni
Asked by: Yonishein
Thank you for your question. Yes, 4% pfa is fine as fixation, although we suggest trying acetone first. You can find the recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-08-21
What is the recommended dilution for this product for fluorescent staining? ~ can i use it at 1:100 dilution?
Asked by: angela94
Thank you for your question.
For immunofluorescence staining, we recommend starting at a 1:50 dilution. Please refer to the full protocol by clicking on the link below.
https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Rated 4 out of
5 by
JM from
Works nicely for IF on Hs68 cellsWorks nicely for IF on Hs68 cells. -SCBT QC
Date published: 2015-04-07
Rated 5 out of
5 by
MS from
Good data with paraffinGood data with paraffin-embedded mouse brain tissue. -SCBT Publication Review
Date published: 2015-03-07
Rated 5 out of
5 by
JM from
Great antibody for WesternGreat antibody for Western, strong band detected in CCD-1064Sk, Hs 732.Sk/Mu, HOS and Hs68 whole cell lysates. -SCBT QC