caspase-8 p18 Antibody (E-8): sc-166320

caspase-8 p18 Antibody (E-8) is an IgG3 κ mouse monoclonal caspase-8 p18 antibody (also designated caspase-8 p18 antibody or CASP8 antibody) that detects p18 subunit and precursor of caspase-8 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. caspase-8 p18 Antibody (E-8) is available as the non-conjugated anti-caspase-8 p18 antibody form, as well as multiple conjugated forms of anti-caspase-8 p18 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, and may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-8 p18 Antibody (E-8) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-8 p18 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-8 p18 Antibody (E-8) Specifications

Alternate Product Name

CASP8

Product Clone Name

E-8

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₃ kappa light chain

Amino Acids

221-236

Epitope Target

caspase-8 p18

Epitope Species

human

Product Reactivity/Detection

p18 subunit and precursor of caspase-8

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

12 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred caspase-8 p18 antibody is caspase-8 p18 Antibody (D-8): sc-5263.