1-800-457-3801

SCBT

COL5A1 Antibody (B-11): sc-166194

4.0(1)
Write a reviewAsk a question

Datasheets
  • COL5A1 Antibody (B-11) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
  • raised against amino acids 251-450 mapping within an extracellular domain of Collagen α1 Type V of human origin
  • recommended for detection of Collagen α1 Type V of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for COL5A1 Antibody (B-11) for WB applications. These reagents are now offered in bundles with COL5A1 Antibody (B-11) (see ordering information below).

    QUICK LINKS

    SEE ALSO...

    The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    COL5A1 Antibody (B-11)

    sc-166194
    200 µg/ml
    $310.00

    COL5A1 Antibody (B-11): m-IgG Fc BP-HRP Bundle

    sc-540041
    200 µg Ab; 10 µg BP
    $348.00

    COL5A1 Antibody (B-11): m-IgG2b BP-HRP Bundle

    sc-549769
    200 µg Ab; 10 µg BP
    $348.00

    How much sample do you recommend loading on the gel for Western blot detection using COL5A1 (B-11): sc-166194?

    Asked by: cjMara
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2024, m_5, d_8, h_6CST
    • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasquestionsanswers, tq_1
    • loc_en_US, sid_166194, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getContent, 133ms
    • QUESTIONS, PRODUCT
    Rated 4 out of 5 by from Single strong bad at correct size in CCDSingle strong bad at correct size in CCD-1064Sk whole cell lysate. -SCBT QC
    Date published: 2015-03-17
    • y_2024, m_5, d_8, h_6
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasreviews, tv_0, tr_1
    • loc_en_US, sid_166194, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getReviews, 14ms
    • REVIEWS, PRODUCT
    COL5A1 Antibody (B-11) is rated 4.0 out of 5 by 1.
    • y_2024, m_5, d_8, h_6
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
    • cp_1, bvpage1
    • co_hasreviews, tv_0, tr_1
    • loc_en_US, sid_166194, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getAggregateRating, 109ms
    • REVIEWS, PRODUCT