DSC2/3 Antibody (7G6): sc-53485

DSC2/3 Antibody (7G6) is an IgG₁ κ mouse monoclonal DSC2/3 antibody (also designated DSC2/3 antibody) that detects the DSC2/3 protein of mouse, rat and human origin by WB, IP and IF. DSC2/3 Antibody (7G6) is available as both the non-conjugated anti-DSC2/3 antibody form, as well as multiple conjugated forms of anti-DSC2/3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Desmogleins are type I membrane proteins that are important for cell adhesion and are expressed in great abundance at the desmosomes, which are adhesive cell junctions. Desmogleins belong to the cadherin family and consist of Dsg1, Dsg2, Dsg3 and Dsg4. The desmosomal cadherin desmocollins DSC1 and DSC3 are also type I membrane proteins that may contribute to epidermal cell positioning by mediating differential adhesiveness between cells that express different isoforms. Alternative splicing gives rise to isoforms A and B of DSC1 and DSC3, which differ in their C-termini. DSC2 exhibits homophilic interactions in solution, and forms heterophilic interactions with Dsg2. DSC2 and DSC1 are present at high levels in the suprabasal skin layers. Dsc2 protein is predominantly localized to specialized adhesion junctions between the cortex and the medulla. DSC3 is expressed in all living epidermal layers as well as in glandular ducts, basal matrix cells and the outer root sheath of hair follicles. DSC3, but not DSC1, is also present in desmosomes of the basal and suprabasal cell layers of other stratified epithelia such as cervix, tongue and esophagus as well as in cells of the basal layer of bladder urothelium and the complex epithelium of trachea.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Genomic organization and amplification of the human desmosomal cadherin genes DSC1 and DSC3, encoding desmocollin types 1 and 3.  |  Whittock, NV., et al. 2000. Biochem Biophys Res Commun. 276: 454-60. PMID: 11027496
2. Molecular interactions between desmosomal cadherins.  |  Syed, SE., et al. 2002. Biochem J. 362: 317-27. PMID: 11853539
3. Genomic organization of mouse desmocollin genes reveals evolutionary conservation.  |  Kljuic, A., et al. 2004. DNA Seq. 15: 148-52. PMID: 15346771
4. Foxn1 is required for tissue assembly and desmosomal cadherin expression in the hair shaft.  |  Johns, SA., et al. 2005. Dev Dyn. 232: 1062-8. PMID: 15739220
5. Desmosome-binding antibody KM48 recognises an extracellular antigen different from desmosomal cadherins Dsg 1-3 and Dsc 1-3.  |  Duhieu, S., et al. 2005. Eur J Dermatol. 15: 80-4. PMID: 15757816
6. Patterns of desmocollin synthesis in human epithelia: immunolocalization of desmocollins 1 and 3 in special epithelia and in cultured cells.  |  Nuber, UA., et al. 1996. Eur J Cell Biol. 71: 1-13. PMID: 8884173

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DSC2/3 Antibody (7G6) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-DSC2/3 Antibody (7G6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DSC2/3 Antibody (7G6) Specifications

Alternate Product Name

DSC2/3

Product Clone Name

7G6

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

Epitope Target

DSC2

Epitope Species

human

Product Reactivity/Detection

DSC2 and DSC3

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

6 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin