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Fibronectin Antibody (EP5): sc-8422
- Fibronectin Antibody (EP5) is a mouse monoclonal IgG1 κ Fibronectin antibody, cited in 296 publications, provided at 200 µg/ml
- raised against a T cell leukemia biopsy of human origin
- Fibronectin Antibody (EP5) is recommended for detection of Fibronectin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Fibronectin Antibody (EP5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Fibronectin (EP5): sc-8422.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Fibronectin Antibody (EP5) for WB and IHC(P) applications. These reagents are now offered in bundles with Fibronectin Antibody (EP5) (see ordering information below).
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Fibronectin Antibody (EP5) is an IgG1 κ mouse monoclonal Fibronectin antibody (also designated FN1 antibody) that detects the Fibronectin protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Fibronectin Antibody (EP5) is available as both the non-conjugated anti-Fibronectin antibody form, as well as multiple conjugated forms of anti-Fibronectin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren′s disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- The distribution of fibronectin, laminin and tetranectin in human breast cancer with special attention to the extracellular matrix. | Christensen, L. 1992. APMIS Suppl. 26: 1-39. PMID: 1576006
- Monoclonal antibody characterization of two distant sites required for function of the central cell-binding domain of fibronectin in cell adhesion, cell migration, and matrix assembly. | Nagai, T., et al. 1991. J Cell Biol. 114: 1295-305. PMID: 1716636
- Fibronectin and viral pathogenesis. | Keski-Oja, J., et al. 1987. Rev Infect Dis. 9 Suppl 4: S404-11. PMID: 3326136
- Monoclonal antibodies that distinguish between human cellular and plasma fibronectin. | Keen, J., et al. 1984. Mol Biol Med. 2: 15-27. PMID: 6083431
- The structure of fibronectin and its role in cellular adhesion. | Akiyama, SK., et al. 1981. J Supramol Struct Cell Biochem. 16: 345-8. PMID: 6273596
- Molecular and biological interactions of fibronectin. | Ruoslahti, E., et al. 1982. J Invest Dermatol. 79 Suppl 1: 65s-68s. PMID: 7045246
- Differential expression of fibronectin splice variants, oncofetal glycosylated fibronectin and laminin isoforms in nodular palmar fibromatosis. | Kosmehl, H., et al. 1995. Pathol Res Pract. 191: 1105-13. PMID: 8822112
- Soluble and insoluble fibronectin increases alveolar epithelial wound healing in vitro. | Garat, C., et al. 1996. Am J Physiol. 271: L844-53. PMID: 8944729
- Expression, localization and alternative splicing pattern of fibronectin messenger RNA in fibrotic human liver and hepatocellular carcinoma. | Matsui, S., et al. 1997. J Hepatol. 27: 843-53. PMID: 9382972
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fibronectin Antibody (EP5) | sc-8422 | 200 µg/ml | $310.00 | |||
Fibronectin Antibody (EP5): m-IgG Fc BP-HRP Bundle | sc-528228 | 200 µg Ab; 10 µg BP | $348.00 | |||
Fibronectin Antibody (EP5): m-IgGκ BP-HRP Bundle | sc-520554 | 200 µg Ab, 40 µg BP | $348.00 | |||
Fibronectin Antibody (EP5): m-IgG1 BP-HRP Bundle | sc-542827 | 200 µg Ab; 20 µg BP | $348.00 | |||
Fibronectin Antibody (EP5) AC | sc-8422 AC | 500 µg/ml, 25% agarose | $408.00 | |||
Fibronectin Antibody (EP5) HRP | sc-8422 HRP | 200 µg/ml | $310.00 | |||
Fibronectin Antibody (EP5) FITC | sc-8422 FITC | 200 µg/ml | $324.00 | |||
Fibronectin Antibody (EP5) PE | sc-8422 PE | 200 µg/ml | $337.00 | |||
Fibronectin Antibody (EP5) Alexa Fluor® 488 | sc-8422 AF488 | 200 µg/ml | $350.00 | |||
Fibronectin Antibody (EP5) Alexa Fluor® 546 | sc-8422 AF546 | 200 µg/ml | $350.00 | |||
Fibronectin Antibody (EP5) Alexa Fluor® 594 | sc-8422 AF594 | 200 µg/ml | $350.00 | |||
Fibronectin Antibody (EP5) Alexa Fluor® 647 | sc-8422 AF647 | 200 µg/ml | $350.00 | |||
Fibronectin Antibody (EP5) Alexa Fluor® 680 | sc-8422 AF680 | 200 µg/ml | $350.00 | |||
Fibronectin Antibody (EP5) Alexa Fluor® 790 | sc-8422 AF790 | 200 µg/ml | $350.00 |