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Fibronectin Antibody (P1H11): sc-18825

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Datasheets
  • Fibronectin Antibody (P1H11) is a mouse monoclonal IgG1 κ, cited in 76 publications, provided at 200 µg/ml
  • raised against cell binding domain of Fibronectin of human origin
  • Anti-Fibronectin Antibody (P1H11) is recommended for detection of 38 kDa and 190 kDa precursor from the A chain of Fibronectin of mouse, rat, human and primate origin by WB, IP, IF and IHC(P)
  • Anti-Fibronectin Antibody (P1H11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also provided azide-free for biological studies, sc-18825 L, 200 µg/0.1 ml
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Fibronectin (P1H11): sc-18825.
  • m-IgGκ BP-HRP is the preferred secondary detection reagent for Fibronectin Antibody (P1H11) for WB and IHC(P) applications. This reagent is now offered in a bundle with Fibronectin Antibody (P1H11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

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Fibronectin Antibody (P1H11) is an IgG1 κ mouse monoclonal Fibronectin antibody that detects 38 kDa and 190 kDa precursor from the A chain of Fibronectin of mouse, rat and human origin by WB, IP, IF and IHC(P). Fibronectin Antibody (P1H11) is available as the non-conjugated anti-Fibronectin antibody form, as well as multiple conjugated forms of anti-Fibronectin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren′s disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Fibronectin Antibody (P1H11)

sc-18825
200 µg/ml
$310.00

Fibronectin Antibody (P1H11): m-IgGκ BP-HRP Bundle

sc-520687
200 µg Ab, 40 µg BP
$348.00

Fibronectin Antibody (P1H11) L

sc-18825 L
200 µg/0.1 ml
$310.00

Fibronectin Antibody (P1H11) AC

sc-18825 AC
500 µg/ml, 25% agarose
$408.00

Fibronectin Antibody (P1H11) HRP

sc-18825 HRP
200 µg/ml
$310.00

Fibronectin Antibody (P1H11) FITC

sc-18825 FITC
200 µg/ml
$324.00

Fibronectin Antibody (P1H11) PE

sc-18825 PE
200 µg/ml
$337.00

Fibronectin Antibody (P1H11) Alexa Fluor® 488

sc-18825 AF488
200 µg/ml
$350.00

Fibronectin Antibody (P1H11) Alexa Fluor® 546

sc-18825 AF546
200 µg/ml
$350.00

Fibronectin Antibody (P1H11) Alexa Fluor® 594

sc-18825 AF594
200 µg/ml
$350.00

Fibronectin Antibody (P1H11) Alexa Fluor® 647

sc-18825 AF647
200 µg/ml
$350.00

Fibronectin Antibody (P1H11) Alexa Fluor® 680

sc-18825 AF680
200 µg/ml
$350.00

Fibronectin Antibody (P1H11) Alexa Fluor® 790

sc-18825 AF790
200 µg/ml
$350.00

Can the Fibronectin (P1H11): sc-18825 mouse monoclonal antibody be used for immunohistochemical staining of mouse tissue?

Asked by: cjMara
Yes. We recommend using our exclusive mouse IgG binding protein conjugated to HRP (sc-516102) in place of a conventional secondary antibody. Alternatively, we recommend using a directly conjugated monoclonal antibody. Please contact our Special Orders department for availability of direct conjugates of Fibronectin (P1H11): sc-18825. Otherwise, see alternative clone, Fibronectin (EP5): sc-8422 for antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 4 out of 5 by from Good for ImmunohistochemistryIt showed to be good for Immunohistochemistry on Paraffin-embedded Human Adrenocortical Carcinoma samples.
Date published: 2019-10-14
Rated 5 out of 5 by from 效果很好效果好,条带单一。找了几家抗体公司,终于找到能孵出条带的抗体了。目的条带在200KDa左右的位置,条带清晰。
Date published: 2018-11-13
Rated 4 out of 5 by from Good productI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 4 out of 5 by from Good for IF on rat frozen liver sectionsRat liver frozen sections were subjected to double-immunofluorescence using Fibronectin (P1H11) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. Fibronectin positive cells were observed clearly by heat-induced citrate buffer retrieval.
Date published: 2018-03-01
Rated 5 out of 5 by from good signalsThis antibody performs well for detecting Fibronectin, clear and clean, recommend to use.
Date published: 2017-05-17
Rated 5 out of 5 by from Strong signal with this antibodyThis monoclonal antibody produced nice signal by WB, IHC(P) and IF. Highly recommended!
Date published: 2017-01-16
Rated 3 out of 5 by from Banding with HepG2 whole cell lysate a littleBanding with HepG2 whole cell lysate a little fuzzy but distinct. -SCBT QC
Date published: 2014-11-29
Rated 5 out of 5 by from IHC worked well with mouse samplesIHC worked well with mouse samples, citrate buffer retrieval. 1:500 dilution. -SCBT Publication Review
Date published: 2012-11-12
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Fibronectin Antibody (P1H11) is rated 4.5 out of 5 by 11.
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