α-dystroglycan Antibody (IIH6): sc-53987

α-dystroglycan Antibody (IIH6) is an IgM κ mouse monoclonal alpha-dystroglycan antibody (also designated DAG1 antibody or Alpha Dystroglycan antibody) that detects the alpha-dystroglycan protein of mouse, rat, human, rabbit and canine origin by WB, IP, IF and IHC(P). α-dystroglycan Antibody (IIH6) is available as both the non-conjugated anti-alpha-dystroglycan antibody, and multiple Alexa Fluor^® conjugates. Dystroglycan (DG) is a cell surface receptor for several extracellular matrix molecules including laminins, Agrin and Perlecan. Dystroglycan function is required for the formation of basement membranes in early development and the organization of Laminin on the cell surface. α-dystroglycan is a membrane- associated, extracellular glycoprotein that is anchored to the cell-membrane by binding to the transmembrane glycoprotein α-dystroglycan to form an α/β-dystroglycan-complex. Additionally, dystroglycan is part of a multimolecular complex, where it associates with dystrophin, at the sarcolemma, to form the dystrophin-associated protein complex, or with utrophin, at the neuromuscular junction, to form the utrophin-associated protein complex. Dystroglycan is also thought to participate in the clustering of nicotinic acetylcholine receptors at the neuromuscular junction.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Chimaeric mice deficient in dystroglycans develop muscular dystrophy and have disrupted myoneural synapses.  |  Côtε, PD., et al. 1999. Nat Genet. 23: 338-42. PMID: 10610181
2. Differential targeting of components of the dystrophin complex to the postsynaptic membrane.  |  Marchand, S., et al. 2001. Eur J Neurosci. 13: 221-9. PMID: 11168526
3. Distinct roles for dystroglycan, beta1 integrin and perlecan in cell surface laminin organization.  |  Henry, MD., et al. 2001. J Cell Sci. 114: 1137-44. PMID: 11228157
4. Expression of dystroglycan complex in satellite cells of dorsal root ganglia.  |  Masaki, T., et al. 2001. Acta Neuropathol. 101: 174-8. PMID: 11271373
5. Syntheses of alpha-dystroglycan derived glycosyl amino acids carrying a novel mannosyl serine/threonine linkage.  |  Seifert, J., et al. 2000. Glycoconj J. 17: 407-23. PMID: 11294507
6. Dystroglycan: a possible mediator for reducing congenital muscular dystrophy?  |  Sciandra, F., et al. 2007. Trends Biotechnol. 25: 262-8. PMID: 17416431
7. Inflammation and response to steroid treatment in limb-girdle muscular dystrophy 2I.  |  Darin, N., et al. 2007. Eur J Paediatr Neurol. 11: 353-7. PMID: 17446099
8. Expression of the murine Pomt1 gene in both the developing brain and adult muscle tissues and its relationship with clinical aspects of Walker-Warburg syndrome.  |  Prados, B., et al. 2007. Am J Pathol. 170: 1659-68. PMID: 17456771
9. Localized treatment with a novel FDA-approved proteasome inhibitor blocks the degradation of dystrophin and dystrophin-associated proteins in mdx mice.  |  Bonuccelli, G., et al. 2007. Cell Cycle. 6: 1242-8. PMID: 17495527

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-dystroglycan Antibody (IIH6) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-α-dystroglycan Antibody (IIH6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-dystroglycan Antibody (IIH6) Specifications

Alternate Product Name

Alpha Dystroglycan

Product Clone Name

IIH6

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgM kappa light chain

Amino Acids

Epitope Target

α-dystroglycan

Epitope Species

rabbit

Product Reactivity/Detection

α-dystroglycan

Species Reactivity/Detection

mouse, rat, human, rabbit, canine

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P)

Vialing Information

Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

30 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Kappa Light Chain Binding Proteins

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

N/A