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CaM Antibody (G-3): sc-137079
- CaM Antibody (G-3) is a mouse monoclonal IgG2a κ CaM antibody, cited in 27 publications, provided at 200 µg/ml
- raised against amino acids 1-149 representing full length CaM I I of human origin
- CaM Antibody (G-3) is recommended for detection of calmodulin and CALML3 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-CaM Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CaM (G-3): sc-137079.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CaM Antibody (G-3) for WB applications. These reagents are now offered in bundles with CaM Antibody (G-3) (see ordering information below).
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CaM Antibody (G-3) is an IgG2a κ mouse monoclonal CaM antibody (also designated CALM1 antibody, CALM2 antibody, CALM3 antibody, CALML3 antibody, pan Calmodulin antibody, or Phosphorylase Kinase Delta antibody) that detects the CaM protein of mouse, rat and human origin by WB, IP, IF and ELISA. CaM Antibody (G-3) is available as both the non-conjugated anti-CaM antibody form, as well as multiple conjugated forms of anti-CaM antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The level of intracellular calcium is tightly regulated in all eukaryotic cells. A modest increase in this level can result in a myriad of physiological responses, most of which are mediated by calmodulin (CaM), the universal calcium sensor. CaM directly modulates the activity of protein kinases and phosphatases, ion channels and nitric oxide synthetases. It is generally involved in such diverse processes as cell proliferation, endocytosis, cellular adhesion, protein turn over and smooth muscle contraction. CaM (calmodulin) is an acidic protein, 148 amino acids in length, with four helix-loop-helix calcium binding domains. In humans, 3 distinct genes have been identified (CALM1, CALM2 and CALM3); each encoding the identical protein. CALML3 (calmodulin-like 3, or calmodulin-related protein NB-1) shares significant sequence identity with CaM and it is suggested that it may competitively bind CaM substrates. Interestingly, CaM has been shown to associate with the carboxy terminus of the dystrophin gene product, implying that it may regulate its activity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- The Merck Frosst Award Lecture 1994. Calmodulin: a versatile calcium mediator protein. | Vogel, HJ. 1994. Biochem Cell Biol. 72: 357-76. PMID: 7605608
- Molecular and structural basis of target recognition by calmodulin. | Crivici, A. and Ikura, M. 1995. Annu Rev Biophys Biomol Struct. 24: 85-116. PMID: 7663132
- Calcium, calmodulin and cell cycle progression. | Takuwa, N., et al. 1995. Cell Signal. 7: 93-104. PMID: 7794690
- Calcium/calmodulin-dependent protein kinases. | Nairn, AC. and Picciotto, MR. 1994. Semin Cancer Biol. 5: 295-303. PMID: 7803766
- Ion channel regulation by calmodulin binding. | Saimi, Y. and Kung, C. 1994. FEBS Lett. 350: 155-8. PMID: 8070555
- Calmodulin is the divalent cation receptor for rapid endocytosis, but not exocytosis, in adrenal chromaffin cells. | Artalejo, CR., et al. 1996. Neuron. 16: 195-205. PMID: 8562084
- Nitric oxide synthase: expression of the endothelial, Ca2+/calmodulin-dependent isoform in human B and T lymphocytes. | Reiling, N., et al. 1996. Eur J Immunol. 26: 511-6. PMID: 8605914
- A new function for adducin. Calcium/calmodulin-regulated capping of the barbed ends of actin filaments. | Kuhlman, PA., et al. 1996. J Biol Chem. 271: 7986-91. PMID: 8626479
- Ca2+-calmodulin binds to the carboxyl-terminal domain of dystrophin. | Anderson, JT., et al. 1996. J Biol Chem. 271: 6605-10. PMID: 8636075
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaM Antibody (G-3) | sc-137079 | 200 µg/ml | $310.00 | |||
CaM Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-528855 | 200 µg Ab; 10 µg BP | $348.00 | |||
CaM Antibody (G-3): m-IgGκ BP-HRP Bundle | sc-521419 | 200 µg Ab, 40 µg BP | $348.00 | |||
CaM Antibody (G-3): m-IgG2a BP-HRP Bundle | sc-547192 | 200 µg Ab; 10 µg BP | $348.00 | |||
CaM Antibody (G-3) AC | sc-137079 AC | 500 µg/ml, 25% agarose | $408.00 | |||
CaM Antibody (G-3) HRP | sc-137079 HRP | 200 µg/ml | $310.00 | |||
CaM Antibody (G-3) FITC | sc-137079 FITC | 200 µg/ml | $324.00 | |||
CaM Antibody (G-3) PE | sc-137079 PE | 200 µg/ml | $337.00 | |||
CaM Antibody (G-3) Alexa Fluor® 488 | sc-137079 AF488 | 200 µg/ml | $350.00 | |||
CaM Antibody (G-3) Alexa Fluor® 546 | sc-137079 AF546 | 200 µg/ml | $350.00 | |||
CaM Antibody (G-3) Alexa Fluor® 594 | sc-137079 AF594 | 200 µg/ml | $350.00 | |||
CaM Antibody (G-3) Alexa Fluor® 647 | sc-137079 AF647 | 200 µg/ml | $350.00 | |||
CaM Antibody (G-3) Alexa Fluor® 680 | sc-137079 AF680 | 200 µg/ml | $350.00 | |||
CaM Antibody (G-3) Alexa Fluor® 790 | sc-137079 AF790 | 200 µg/ml | $350.00 |