Cytokeratin 20 Antibody (E-9): sc-271183

Cytokeratin 20 Antibody (E-9) is an IgG₁ λ mouse monoclonal Cytokeratin 20 antibody (also designated KRT20 antibody, or Keratin 20 antibody) that detects the Cytokeratin 20 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Cytokeratin 20 Antibody (E-9) is available as both the non-conjugated anti-Cytokeratin 20 antibody form, as well as multiple conjugated forms of anti-Cytokeratin 20 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epi-thelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition, they aid in the characterization of malignant tumors. Cytokeratin 20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract, and Cytokeratin 20 is a useful marker of pancreatic and colorectal cancer. Cytokeratin 20 is also helpful in distinguishing different types of highly related carcinomas, such as renal oncocytomas from renal cell carcinomas.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Characterization of cytokeratin 20 expression in pancreatic and colorectal cancer.  |  Wildi, S., et al. 1999. Clin Cancer Res. 5: 2840-7. PMID: 10537351
2. Merkel cell carcinoma can be distinguished from metastatic small cell carcinoma using antibodies to cytokeratin 20 and thyroid transcription factor 1.  |  Leech, SN., et al. 2001. J Clin Pathol. 54: 727-9. PMID: 11533085
3. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary.  |  Wauters, CC., et al. 1995. Hum Pathol. 26: 852-5. PMID: 7543441
4. Cytokeratin expression in normal and (pre)malignant head and neck epithelia: an overview.  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
5. The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas.  |  Moll, R., et al. 1993. Differentiation. 53: 75-93. PMID: 8359595
6. Serum and tissue distribution of a fragment of cytokeratin 19 (cyfra 21-1) in lung cancer patients.  |  Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
7. Keratins and the skin.  |  Fuchs, E. 1995. Annu Rev Cell Dev Biol. 11: 123-53. PMID: 8689554
8. Functional inactivation of p53 by antisense RNA induces invasive ability of lung carcinoma cells and downregulates cytokeratin synthesis.  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
9. Cytokeratin expression, fibrillar organization, and subtle function in liver cells.  |  Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 20 Antibody (E-9) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 20 Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 20 Antibody (E-9) Specifications

Alternate Product Name

KRT20

Product Clone Name

E-9

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ lambda light chain

Amino Acids

39-74

Epitope Target

Cytokeratin 20

Epitope Species

human

Product Reactivity/Detection

Cytokeratin 20

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

3 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin