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pan-Cytokeratin Antibody (K20): sc-53402

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Datasheets
  • pan-Cytokeratin Antibody (K20) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
  • raised against hair keratin of human origin
  • recommended for detection of broad range of Cytokeratin proteins of human origin by WB, IP, IF and IHC(P)
  • See pan-Cytokeratin (C11): sc-8018 for pan-Cytokeratin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for pan-Cytokeratin Antibody (K20) for WB and IHC(P) applications. These reagents are now offered in bundles with pan-Cytokeratin Antibody (K20) (see ordering information below).

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Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

pan-Cytokeratin Antibody (K20)

sc-53402
200 µg/ml
$310.00

pan-Cytokeratin Antibody (K20): m-IgG Fc BP-HRP Bundle

sc-536956
200 µg Ab; 10 µg BP
$348.00

pan-Cytokeratin Antibody (K20): m-IgGκ BP-HRP Bundle

sc-534133
200 µg Ab; 40 µg BP
$348.00

Can pan-Cytokeratin (K20): sc-53402 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, pan-Cytokeratin (K20): sc-53402 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Worked with transfected lysate by WB withWorked with transfected lysate by WB with some background -SCBT QC
Date published: 2015-03-21
Rated 4 out of 5 by from Good specificity and signal strength withGood specificity and signal strength with faint background -SCBT QC
Date published: 2015-03-17
Rated 4 out of 5 by from Satisfactory Western blot analysis of panSatisfactory Western blot analysis of pan-Cytokeratin expression in LS1034 and A-431 whole cell lysates. -SCBT QC
Date published: 2013-11-07
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pan-Cytokeratin Antibody (K20) is rated 4.0 out of 5 by 3.
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