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GST Antibody (B-14): sc-138
- GST Antibody (B-14) is a mouse monoclonal IgG1 κ GST antibody, cited in 1,358 publications, provided at 200 µg/ml
- raised against the 26 kDa GST specific domain of a fusion protein encoded by a pGEX.3X recombinant vector
- GST Antibody (B-14) is recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB and IP; specifically designed to be used with GST expression vectors such as pGEX.3X and pGEX.2T
- Anti-GST Antibody (B-14) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to biotin for WB, IHC(P) and ELISA; and to TRITC for IF, IHC(P) and FCM
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GST (B-14): sc-138.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GST Antibody (B-14) for WB applications. These reagents are now offered in bundles with GST Antibody (B-14) (see ordering information below).
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GST Antibody (B-14) is an IgG1 κ mouse monoclonal GST antibody (also designated Glutathione S-Transferase antibody) that detects the GST protein of Schistosoma japonicum origin by WB and IP. GST Antibody (B-14) is available as both the non-conjugated anti-GST antibody form, as well as multiple conjugated forms of anti-GST antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathione-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Expression of GST-fused kinase domain of human Csk homologous kinase from Pichia pastoris facilitates easy purification. | Murthy, TV. 2004. Biotechnol Lett. 26: 443-9. PMID: 15104145
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. | Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
- Cloning, gene sequencing, and expression of the small molecular mass ubiquinone-binding protein of mitochondrial ubiquinol-cytochrome c reductase. | Yu, L., et al. 1995. J Biol Chem. 270: 25634-8. PMID: 7592738
- Expression of small regions of equine herpesvirus 1 glycoprotein C in Escherichia coli. | Crabb, BS. and Studdert, MJ. 1995. Vet Microbiol. 46: 181-91. PMID: 8545955
- Assessment of antigenicity and genetic variation of glycoprotein B of murine cytomegalovirus. | Xu, J., et al. 1996. J Gen Virol. 77 (Pt 1): 49-59. PMID: 8558128
- Matrilysin: expression, purification, and characterization. | Soler, D., et al. 1995. J Protein Chem. 14: 511-20. PMID: 8561847
- Functional expression of subunit IV of Rhodobacter sphaeroides cytochrome b-c1 complex and reconstitution of recombinant protein with three-subunit core complex. | Chen, YR., et al. 1996. J Biol Chem. 271: 2057-62. PMID: 8567659
- Functional comparison of native and recombinant human salivary histatin 1. | Driscoll, J., et al. 1995. J Dent Res. 74: 1837-44. PMID: 8600179
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GST Antibody (B-14) | sc-138 | 200 µg/ml | $310.00 | |||
GST Antibody (B-14): m-IgG Fc BP-HRP Bundle | sc-528100 | 200 µg Ab; 10 µg BP | $348.00 | |||
GST Antibody (B-14): m-IgGκ BP-HRP Bundle | sc-520398 | 200 µg Ab, 40 µg BP | $348.00 | |||
GST Antibody (B-14): m-IgG1 BP-HRP Bundle | sc-542758 | 200 µg Ab; 20 µg BP | $348.00 | |||
GST Antibody (B-14) AC | sc-138 AC | 500 µg/ml, 25% agarose | $408.00 | |||
GST Antibody (B-14) HRP | sc-138 HRP | 200 µg/ml | $310.00 | |||
GST Antibody (B-14) FITC | sc-138 FITC | 200 µg/ml | $324.00 | |||
GST Antibody (B-14) PE | sc-138 PE | 200 µg/ml | $337.00 | |||
GST Antibody (B-14) Alexa Fluor® 488 | sc-138 AF488 | 200 µg/ml | $350.00 | |||
GST Antibody (B-14) Alexa Fluor® 546 | sc-138 AF546 | 200 µg/ml | $350.00 | |||
GST Antibody (B-14) Alexa Fluor® 594 | sc-138 AF594 | 200 µg/ml | $350.00 | |||
GST Antibody (B-14) Alexa Fluor® 647 | sc-138 AF647 | 200 µg/ml | $350.00 | |||
GST Antibody (B-14) Alexa Fluor® 680 | sc-138 AF680 | 200 µg/ml | $350.00 | |||
GST Antibody (B-14) Alexa Fluor® 790 | sc-138 AF790 | 200 µg/ml | $350.00 | |||
GST Antibody (B-14) B | sc-138 B | 200 µg/ml | $314.00 | |||
GST Antibody (B-14) TRITC | sc-138 TRITC | 200 µg/ml | $314.00 |