granzyme B Antibody (2C5): sc-8022

granzyme B Antibody (2C5) is an IgG_2a κ mouse monoclonal granzyme B antibody (also designated GRAB antibody, Granzyme B antibody, T-Cell Serine Protease 1-3E antibody, Cathepsin G-Like 1 antibody, CTSGL1 antibody, CGL1 antibody, SECT antibody, HLP antibody, Cytotoxic T-Lymphocyte Proteinase 2 antibody, Cytotoxic Serine Protease B antibody, Human Lymphocyte Protein antibody, Fragmentin 2 antibody, CTLA1 antibody, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1 antibody, Lymphocyte Protease antibody, Granzyme 2 antibody, CTLA-1 antibody, or GRB antibody) that detects the granzyme B protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. granzyme B Antibody (2C5) is available as both the non-conjugated anti-granzyme B antibody form, as well as multiple conjugated forms of anti-granzyme B antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Granzyme A and granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways. Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly (ADP ribose) polymerase). Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.

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References:

1. Granzyme A initiates an alternative pathway for granule-mediated apoptosis.  |  Shresta, S., et al. 1999. Immunity. 10: 595-605. PMID: 10367905
2. Dipeptidyl peptidase I is required for the processing and activation of granzymes A and B in vivo.  |  Pham, CT. and Ley, TJ. 1999. Proc Natl Acad Sci U S A. 96: 8627-32. PMID: 10411926
3. Granzyme H destroys the function of critical adenoviral proteins required for viral DNA replication and granzyme B inhibition.  |  Andrade, F., et al. 2007. EMBO J. 26: 2148-57. PMID: 17363894
4. Hepatocellular carcinoma cell supernatants increase expansion and function of CD4(+)CD25(+) regulatory T cells.  |  Cao, M., et al. 2007. Lab Invest. 87: 582-90. PMID: 17372588
5. Cloning and chromosomal assignment of a human cDNA encoding a T cell- and natural killer cell-specific trypsin-like serine protease.  |  Gershenfeld, HK., et al. 1988. Proc Natl Acad Sci U S A. 85: 1184-8. PMID: 3257574
6. Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells.  |  Shresta, S., et al. 1995. Proc Natl Acad Sci U S A. 92: 5679-83. PMID: 7777569
7. Localization of granzyme B in the nucleus. A putative role in the mechanism of cytotoxic lymphocyte-mediated apoptosis.  |  Trapani, JA., et al. 1996. J Biol Chem. 271: 4127-33. PMID: 8626751
8. Cytotoxic T lymphocyte-assisted suicide. Caspase 3 activation is primarily the result of the direct action of granzyme B.  |  Atkinson, EA., et al. 1998. J Biol Chem. 273: 21261-6. PMID: 9694885
9. Efficient nuclear targeting of granzyme B and the nuclear consequences of apoptosis induced by granzyme B and perforin are caspase-dependent, but cell death is caspase-independent.  |  Trapani, JA., et al. 1998. J Biol Chem. 273: 27934-8. PMID: 9774406

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-granzyme B Antibody (2C5) has 52 citations in a variety of scientific publications.

Primary antibodies like Anti-granzyme B Antibody (2C5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

granzyme B Antibody (2C5) Specifications

Alternate Product Name

GZMB

Product Clone Name

2C5

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

1-247

Epitope Target

granzyme B

Epitope Species

human

Product Reactivity/Detection

granzyme B

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

52 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

• m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
• m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
• m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
• m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
• m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
• m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
• m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
• m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
• m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin