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ICAD Antibody (F-8): sc-17816
- ICAD Antibody (F-8) is a mouse monoclonal IgG2a κ ICAD antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-331 representing full length ICAD of human origin
- ICAD Antibody (F-8) is recommended for detection of ICAD of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ICAD Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ICAD (F-8): sc-17816.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ICAD Antibody (F-8) for WB and IHC(P) applications. These reagents are now offered in bundles with ICAD Antibody (F-8) (see ordering information below).
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ICAD Antibody (F-8) is an IgG2a κ mouse monoclonal ICAD antibody (also designated ICAD antibody) that detects the ICAD protein of human origin by WB, IP, IF, IHC(P) and ELISA. ICAD Antibody (F-8) is available as both the non-conjugated anti-ICAD antibody form, as well as multiple conjugated forms of anti-ICAD antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Human plasma gelsolin binds to fibronectin. | Lind, SE. and Janmey, PA. 1984. J Biol Chem. 259: 13262-6. PMID: 6092370
- Mch3, a novel human apoptotic cysteine protease highly related to CPP32. | Fernandes-Alnemri, T., et al. 1995. Cancer Res. 55: 6045-52. PMID: 8521391
- Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis. | Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
- Lamin proteolysis facilitates nuclear events during apoptosis. | Rao, L., et al. 1996. J Cell Biol. 135: 1441-55. PMID: 8978814
- DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. | Liu, X., et al. 1997. Cell. 89: 175-84. PMID: 9108473
- Caspase-3-generated fragment of gelsolin: effector of morphological change in apoptosis. | Kothakota, S., et al. 1997. Science. 278: 294-8. PMID: 9323209
- Caspases: intracellular signaling by proteolysis. | Salvesen, GS. and Dixit, VM. 1997. Cell. 91: 443-6. PMID: 9390553
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ICAD Antibody (F-8) | sc-17816 | 200 µg/ml | $310.00 | |||
ICAD Antibody (F-8): m-IgG Fc BP-HRP Bundle | sc-528325 | 200 µg Ab; 10 µg BP | $348.00 | |||
ICAD Antibody (F-8): m-IgGκ BP-HRP Bundle | sc-520671 | 200 µg Ab, 40 µg BP | $348.00 | |||
ICAD Antibody (F-8): m-IgG2a BP-HRP Bundle | sc-547072 | 200 µg Ab; 10 µg BP | $348.00 | |||
ICAD Antibody (F-8) AC | sc-17816 AC | 500 µg/ml, 25% agarose | $408.00 | |||
ICAD Antibody (F-8) HRP | sc-17816 HRP | 200 µg/ml | $310.00 | |||
ICAD Antibody (F-8) FITC | sc-17816 FITC | 200 µg/ml | $324.00 | |||
ICAD Antibody (F-8) PE | sc-17816 PE | 200 µg/ml | $337.00 | |||
ICAD Antibody (F-8) Alexa Fluor® 488 | sc-17816 AF488 | 200 µg/ml | $350.00 | |||
ICAD Antibody (F-8) Alexa Fluor® 546 | sc-17816 AF546 | 200 µg/ml | $350.00 | |||
ICAD Antibody (F-8) Alexa Fluor® 594 | sc-17816 AF594 | 200 µg/ml | $350.00 | |||
ICAD Antibody (F-8) Alexa Fluor® 647 | sc-17816 AF647 | 200 µg/ml | $350.00 | |||
ICAD Antibody (F-8) Alexa Fluor® 680 | sc-17816 AF680 | 200 µg/ml | $350.00 | |||
ICAD Antibody (F-8) Alexa Fluor® 790 | sc-17816 AF790 | 200 µg/ml | $350.00 |