BUB1 Antibody (B-3): sc-365685

BUB1 Antibody (B-3) is an IgG₃ κ mouse monoclonal BUB1 antibody (also designated BUB1 antibody) that detects the BUB1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. BUB1 Antibody (B-3) is available as the non-conjugated anti-BUB1 antibody. Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.

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References:

1. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC.  |  Chan, GK., et al. 1999. J Cell Biol. 146: 941-54. PMID: 10477750
2. BUBR1 phosphorylation is regulated during mitotic checkpoint activation.  |  Li, W., et al. 1999. Cell Growth Differ. 10: 769-75. PMID: 10593653
3. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis.  |  Kalitsis, P., et al. 2000. Genes Dev. 14: 2277-82. PMID: 10995385
4. CENP-E as an essential component of the mitotic checkpoint in vitro.  |  Abrieu, A., et al. 2000. Cell. 102: 817-26. PMID: 11030625
5. Identification of a novel gene--SSK1--in human endothelial cells exposed to shear stress.  |  Donadelli, R., et al. 1998. Biochem Biophys Res Commun. 246: 881-7. PMID: 9618306
6. The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis.  |  Jablonski, SA., et al. 1998. Chromosoma. 107: 386-96. PMID: 9914370

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BUB1 Antibody (B-3) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-BUB1 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BUB1 Antibody (B-3) Specifications

Alternate Product Name

BUB1

Product Clone Name

B-3

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₃ kappa light chain

Amino Acids

786-1085

Epitope Target

BUB1

Epitope Species

human

Product Reactivity/Detection

BUB1

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

8 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG₃ Binding Proteins

• m-IgG₃ BP-HRP: sc-533670 (Mouse IgG₃ binding protein conjugated to horseradish peroxidase)
• m-IgG₃ BP-B: sc-533672 (Mouse IgG₃ binding protein conjugated to biotin)
• m-IgG₃ BP-FITC: sc-533671 (Mouse IgG₃ binding protein conjugated to fluorescein isothiocyanate)
• m-IgG₃ BP-CFL 488: sc-533673 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 488)
• m-IgG₃ BP-CFL 555: sc-533674 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 555)
• m-IgG₃ BP-CFL 594: sc-533675 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 594)
• m-IgG₃ BP-CFL 647: sc-533676 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 647)
• m-IgG₃ BP-CFL 680: sc-533677 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 680)
• m-IgG₃ BP-CFL 790: sc-533678 (Mouse IgG₃ binding protein conjugated to CruzFluor™ 790)

• m-IgGκ BP-HRP: sc-516102 (Mouse IgGκ light chain binding protein conjugated to horseradish peroxidase)
• m-IgGκ BP-B: sc-516142 (Mouse IgGκ light chain binding protein conjugated to biotin)
• m-IgGκ BP-FITC: sc-516140 (Mouse IgGκ light chain binding protein conjugated to fluorescein isothiocyanate)
• m-IgGκ BP-PE: sc-516141 (Mouse IgGκ light chain binding protein conjugated to phycoerythrin)
• m-IgGκ BP-CFL 488: sc-516176 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 488)
• m-IgGκ BP-CFL 555: sc-516177 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 555)
• m-IgGκ BP-CFL 594: sc-516178 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 594)
• m-IgGκ BP-CFL 647: sc-516179 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 647)
• m-IgGκ BP-CFL 680: sc-516180 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 680)
• m-IgGκ BP-CFL 790: sc-516181 (Mouse IgGκ light chain binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

N/A