Click on image or enlarge button to enlarge
Cdc25A Antibody (5H51): sc-70824
- Cdc25A Antibody (5H51) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
- raised against purified recombinant Cdc25A
- recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF and IHC(P)
- See Cdc25A (F-6): sc-7389 for Cdc25A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc25A Antibody (5H51) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc25A Antibody (5H51) (see ordering information below).
The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by Tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while Tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 Tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a Tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other Tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc25A Antibody (5H51) | sc-70824 | 200 µg/ml | $310.00 | |||
Cdc25A Antibody (5H51): m-IgGκ BP-HRP Bundle | sc-534400 | 200 µg Ab; 40 µg BP | $348.00 | |||
Cdc25A Antibody (5H51): m-IgG2a BP-HRP Bundle | sc-546188 | 200 µg Ab; 10 µg BP | $348.00 |