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Cdc42 Antibody (B-8): sc-8401
- Cdc42 Antibody (B-8) is a mouse monoclonal IgG3 κ Cdc42 antibody, cited in 179 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 166-182 at the C-terminus of Cdc42 of human origin
- recommended for detection of Cdc42 of mouse, rat, human, Drosophila and Xenopus origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc42 (B-8): sc-8401.
- m-IgG3 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc42 Antibody (B-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc42 Antibody (B-8) (see ordering information below).
Cdc42 Antibody (B-8) is an IgG3 κ mouse monoclonal Cdc42 antibody (also designated Cell division control protein 42 homolog antibody, G25K GTP-binding protein antibody, or CDC42 antibody) that detects the Cdc42 protein of mouse, rat, human and d, x origin by WB, IP, IF, IHC(P) and ELISA. Cdc42 Antibody (B-8) is available as both the non-conjugated anti-Cdc42 antibody form, as well as multiple conjugated forms of anti-Cdc42 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The superfamily of GTP-binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of diverse biological activities involving various aspects of cell growth and division. One mammalian member of the family, Cdc42, has an amino acid sequence that is similar to those of various members of the Ras superfamily proteins, including N-, K- and H-Ras, Rho proteins and the Rac proteins. On the basis of in vitro phosphorylation studies, it has been suggested that human Cdc42 may function in the signaling pathway of the EGF receptor or related growth factor receptor protein kinases. The Dbl oncogene has been shown to specifically catalyze dissociation of GDP from human Cdc42.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. | Hart, MJ., et al. 1991. Nature. 354: 311-4. PMID: 1956381
- The cellular functions of small GTP-binding proteins. | Hall, A. 1990. Science. 249: 635-40. PMID: 2116664
- Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42. | Munemitsu, S., et al. 1990. Mol Cell Biol. 10: 5977-82. PMID: 2122236
- The GTPase superfamily: a conserved switch for diverse cell functions. | Bourne, HR., et al. 1990. Nature. 348: 125-32. PMID: 2122258
- Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42. | Shinjo, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9853-7. PMID: 2124704
- Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity. | Johnson, DI. and Pringle, JR. 1990. J Cell Biol. 111: 143-52. PMID: 2164028
- CDC42 and CDC43, two additional genes involved in budding and the establishment of cell polarity in the yeast Saccharomyces cerevisiae. | Adams, AE., et al. 1990. J Cell Biol. 111: 131-42. PMID: 2195038
- Purification of the major GTP-binding proteins from human placental membranes. | Evans, T., et al. 1986. J Biol Chem. 261: 7052-9. PMID: 3084494
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc42 Antibody (B-8) | sc-8401 | 200 µg/ml | $310.00 | |||
Cdc42 Antibody (B-8): m-IgGκ BP-HRP Bundle | sc-520547 | 200 µg Ab, 40 µg BP | $348.00 | |||
Cdc42 Antibody (B-8): m-IgG3 BP-HRP Bundle | sc-550330 | 200 µg Ab; 40 µg BP | $348.00 | |||
Cdc42 Antibody (B-8) AC | sc-8401 AC | 500 µg/ml, 25% agarose | $408.00 | |||
Cdc42 Antibody (B-8) Alexa Fluor® 488 | sc-8401 AF488 | 200 µg/ml | $350.00 | |||
Cdc42 Antibody (B-8) Alexa Fluor® 647 | sc-8401 AF647 | 200 µg/ml | $350.00 | |||
Cdc42 Antibody (B-8) FITC | sc-8401 FITC | 200 µg/ml | $324.00 | |||
Cdc42 Antibody (B-8) HRP | sc-8401 HRP | 200 µg/ml | $310.00 | |||
Cdc42 (B-8) Neutralizing Peptide | sc-8401 P | 100 µg/0.5 ml | $67.00 | |||
Cdc42 Antibody (B-8) PE | sc-8401 PE | 200 µg/ml | $337.00 |